{"created":"2023-05-15T16:32:08.035151+00:00","id":3857,"links":{},"metadata":{"_buckets":{"deposit":"3233c98f-8cfa-4c83-a2e2-5958af8e9bc4"},"_deposit":{"created_by":6,"id":"3857","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"3857"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00003857","sets":["10:22:24"]},"author_link":["115521","115520"],"item_5_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"フリーズドライ保存した多血小板血漿は骨再生に有用である"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-02-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"178","bibliographicPageStart":"172","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"Archives of Oral Biology"}]}]},"item_5_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-02-01"}]},"item_5_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Nagasaki University (長崎大学)"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17301","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. Design: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at ?20 °C for one month. It was then rehydrated with an equal or 1/3 amount of distilled water (×1FD-PRP, ×3FD-PRP, respectively), and we assessed its gelation properties and the release of growth factors (PDGF-BB, TGF-β1, and VEGF). We also examined the bone forming ability with onlay-grafting on mice calvaria using β-TCP granules as a scaffold. Results: FD-PRP showed comparable gelation as f-PRP. In terms of growth factor release, × 1FD-PRP released identical concentrations of PDGF-BB and TGF-β1 to f-PRP, while ×3FD-PRP released approximately 3-fold concentrations when compared with f-PRP. In vivo, ×1FD-PRP promoted identical levels of the bone formation as f-PRP, and ×3FD-PRP induced more abundant bone formation. Conclusions: \nThese results suggest that f-PRP can be stored without functional loss by freeze-drying and the concentration of PRP may improve its efficacy in bone engineering.","subitem_description_type":"Abstract"}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博(医歯薬)甲第913号 学位授与年月日:平成29年2月1日","subitem_description_type":"Other"},{"subitem_description":"Author: Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina","subitem_description_type":"Other"},{"subitem_description":"Citation: Archives of Oral Biology, 73, pp.172-178; 2017","subitem_description_type":"Other"}]},"item_5_description_69":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nagasaki University (長崎大学), 博士(歯学) (2017-02-01)","subitem_description_type":"Other"}]},"item_5_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医歯薬第913号"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"115521","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakatani, Yuya"}]}]},"item_5_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_5_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.archoralbio.2016.10.006","subitem_relation_type_select":"DOI"}}]},"item_5_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2016 Elsevier Ltd. All rights reserved."}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00039969","subitem_source_identifier_type":"ISSN"}]},"item_5_text_67":{"attribute_name":"学位の種類","attribute_value_mlt":[{"subitem_text_value":"課程博士"}]},"item_5_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中谷, 佑哉"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-01"}],"displaytype":"detail","filename":"ISYK913_Nakatani.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISYK913_Nakatani.pdf","objectType":"fulltext","url":"https://nagasaki-u.repo.nii.ac.jp/record/3857/files/ISYK913_Nakatani.pdf"},"version_id":"e9908738-a6ec-49c4-9e83-53dd6873a62b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Platelet-rich plasma","subitem_subject_scheme":"Other"},{"subitem_subject":"Freeze-Dry","subitem_subject_scheme":"Other"},{"subitem_subject":"Long storage","subitem_subject_scheme":"Other"},{"subitem_subject":"Super-concentrated platelets","subitem_subject_scheme":"Other"},{"subitem_subject":"Bone regeneration","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Efficacy of freeze-dried platelet-rich plasma in bone engineering","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficacy of freeze-dried platelet-rich plasma in bone engineering","subitem_title_language":"en"}]},"item_type_id":"5","owner":"6","path":["24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-02-06"},"publish_date":"2017-02-06","publish_status":"0","recid":"3857","relation_version_is_last":true,"title":["Efficacy of freeze-dried platelet-rich plasma in bone engineering"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-08-23T04:55:21.664426+00:00"}