{"created":"2023-05-15T16:32:16.788121+00:00","id":4060,"links":{},"metadata":{"_buckets":{"deposit":"acc6df1e-7c14-4b11-ad2c-2c7255b10a51"},"_deposit":{"created_by":2,"id":"4060","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4060"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004060","sets":["73:74"]},"author_link":["16203","16210","16208","16202","16209","16207","16211","16204","16205","16212","16206"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-02-04","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"22","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Frontiers in Cellular Neuroscience"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Calpains are ubiquitous proteases involved in cell proliferation, adhesion and motility. In the brain, calpains have been associated with neuronal damage in both acute and neurodegenerative disorders, but their physiological function in the nervous system remains elusive. During brain ischemia, there is a large increase in the levels of intracellular calcium, leading to the activation of calpains. Inhibition of these proteases has been shown to reduce neuronal death in a variety of stroke models. On the other hand, after stroke, neural stem cells (NSC) increase their proliferation and newly formed neuroblasts migrate towards the site of injury. However, the process of forming new neurons after injury is not efficient and finding ways to improve it may help with recovery after lesion. Understanding the role of calpains in the process of neurogenesis may therefore open a new window for the treatment of stroke. We investigated the involvement of calpains in NSC proliferation and neuroblast migration in two highly neurogenic regions in the mouse brain, the dentate gyrus (DG) and the subventricular zone (SVZ). We used mice that lack calpastatin, the endogenous calpain inhibitor, and calpains were also modulated directly, using calpeptin, a pharmacological calpain inhibitor. Calpastatin deletion impaired both NSC proliferation and neuroblast migration. Calpain inhibition increased NSC proliferation, migration speed and migration distance in cells from the SVZ. Overall, our work suggests that calpains are important for neurogenesis and encourages further research on their neurogenic role. Prospective therapies targeting calpain activity may improve the formation of new neurons following stroke, in addition to affording neuroprotection.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Frontiers in Cellular Neuroscience, 9, 22; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Research Foundation"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3389/fncel.2015.00022","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2015 Machado, Morte, Carreira, Azevedo, Takano, Iwata, Saido, Asmussen, Horwitz, Carvalho and Araujo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"16625102","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Machado, Vanessa M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morte, Maria Ines"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Carreira, Bruno Pereira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Azevedo, Maria M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takano, Jiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwata, Nobuhisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saido, Takaomi Comings"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asmussen, Hannelore"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Horwitz, Alan Rick"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Carvalho, Caetana Monteiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Araujo, Ines Maria"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"Fncel9_22.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Fncel9_22.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4060/files/Fncel9_22.pdf"},"version_id":"151510ff-007e-41b2-abc7-86cae316fea5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Calpains","subitem_subject_scheme":"Other"},{"subitem_subject":"Calpastatin","subitem_subject_scheme":"Other"},{"subitem_subject":"Hippocampus","subitem_subject_scheme":"Other"},{"subitem_subject":"Migration","subitem_subject_scheme":"Other"},{"subitem_subject":"Neurogenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"Proliferation","subitem_subject_scheme":"Other"},{"subitem_subject":"Stroke","subitem_subject_scheme":"Other"},{"subitem_subject":"Subventricular zone","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Involvement of calpains in adult neurogenesis: implications for stroke","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Involvement of calpains in adult neurogenesis: implications for stroke"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-31"},"publish_date":"2015-03-31","publish_status":"0","recid":"4060","relation_version_is_last":true,"title":["Involvement of calpains in adult neurogenesis: implications for stroke"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:26:20.717942+00:00"}