{"created":"2023-05-15T16:32:21.627309+00:00","id":4174,"links":{},"metadata":{"_buckets":{"deposit":"7bff8070-bd7a-498b-b323-af721c77ab1a"},"_deposit":{"created_by":2,"id":"4174","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4174"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004174","sets":["29:30"]},"author_link":["16722","16723"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-08-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"810","bibliographicPageStart":"805","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Analytical Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Multiplex ligation-dependent probe amplification (MLPA) is a widely used technique for detecting genomic structural variants. The technique is based on hybridization and ligation, followed by amplification of the ligation products. Therefore, ligation is considered a fundamental process that determines the feasibility and fidelity of MLPA. However, despite the widespread use of this technique, its reaction mechanism has not been fully analyzed. Herein, we describe a ligation-independent pathway for MLPA and introduce a ligation-independent probe amplification system that can be used to obtain amplified products without the hybridization and ligation processes. Fragment analysis revealed that the ligation-independent pathway is functional and that the capacity to discriminate single nucleotides with MLPA does not depend on ligation. These findings indicate that the feasibility and fidelity of MLPA do not rely on ligation.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Analytical Sciences, 30(8), pp.805-810; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本分析化学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2116/analsci.30.805","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 by The Japan Society for Analytical Chemistry"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09106340","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13482246","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Japan Society for Analytical Chemistry"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uno, Naoki"}],"nameIdentifiers":[{"nameIdentifier":"16722","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagihara, Katsunori"}],"nameIdentifiers":[{"nameIdentifier":"16723","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"AnaSci30_805.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AnaSci30_805.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4174/files/AnaSci30_805.pdf"},"version_id":"0258a992-e7b7-4c11-98e3-88e0b8f5afae"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fragment analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"Helicase-dependent amplification","subitem_subject_scheme":"Other"},{"subitem_subject":"Ligation","subitem_subject_scheme":"Other"},{"subitem_subject":"MLPA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ligation-Independent Mechanism of Multiplex Ligation-Dependent Probe Amplification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ligation-Independent Mechanism of Multiplex Ligation-Dependent Probe Amplification"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-12"},"publish_date":"2015-05-12","publish_status":"0","recid":"4174","relation_version_is_last":true,"title":["Ligation-Independent Mechanism of Multiplex Ligation-Dependent Probe Amplification"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:24:58.449300+00:00"}