{"created":"2023-05-15T16:32:22.353603+00:00","id":4191,"links":{},"metadata":{"_buckets":{"deposit":"6a7a0775-c6e8-4026-838f-474fcdf5a341"},"_deposit":{"created_by":2,"id":"4191","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4191"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004191","sets":["8:9"]},"author_link":["16867","16870","16868","16869","16865","16864","16863","16866","16862"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"1106","bibliographicPageStart":"1096","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"Arthritis & Rheumatology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective To investigate whether human T lymphotropic virus type I (HTLV-I) directly infects salivary gland epithelial cells (SGECs) and induces the niche of the salivary glands in patients with Sjogren's syndrome (SS). Methods SGECs were cultured with the HTLV-I-producing CD4+ T cell line HCT-5 or with Jurkat cells. Antibody arrays, immunofluorescence analysis, and enzyme-linked immunosorbent assay (ELISA) were used to determine the profiles of inflammation-related molecules, and the profiles of apoptosis-related molecules were determined by antibody array and immunofluorescence analysis. The presence of HTLV-I-related molecules was assessed by immunofluorescence analysis and in situ polymerase chain reaction. Apoptosis of SGECs was evaluated by TUNEL staining. Results Among the SGECs, 7.8 ± 1.3% (mean ± SD) were positive for HTLV-I-related proteins after 96-hour coculture with HCT-5 cells. Nuclear NF-κB p65 was also detected in 10% of the SGECs. The presence of HTLV-I proviral DNA in SGECs after coculture with HCT-5 cells was detected by in situ polymerase chain reaction. After coculture of SGECs with HCT-5, the expression of cytokines and chemokines, including soluble intercellular adhesion molecule 1, RANTES, and interferon γ-induced protein 10 kd (IP-10/CXCL10) was increased in a time-dependent manner. The expression of proapoptotic molecules (e.g., cytochrome c and Fas) and antiapoptotic molecules (e.g., Bcl-2, Heme oxygenase 2, and Hsp27) was increased in the SGECs cocultured with HCT-5, showing that apoptosis of SGECs was not detected after coculture with HCT-5 or Jurkat cells. Conclusion HTLV-I is thought to infect SGECs and alter their cellular functions. These changes may induce the niche of SS and contribute to the development of SS in anti-HTLV-I antibody-positive individuals.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Arthritis & Rheumatology, 67(4), pp.1096-1106; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley and Sons Ltd"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/art.39009","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2015 by the American College of Rheumatology."},{"subitem_rights":"This is the accepted version of the following article: Arthritis and Rheumatology, 67(4), pp.1096-1106; 2015, which has been published in final form at http://dx.doi.org/10.1002/art.39009"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"23265191","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakamura, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Yoshiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto-Fukuda, Tomomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Horai, Yoshiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Yoshikazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arima, Kazuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tatsufumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koji, Takehiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Atsushi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"ArtRhe67_1096.pdf","filesize":[{"value":"3.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ArtRhe67_1096.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4191/files/ArtRhe67_1096.pdf"},"version_id":"2646b9d1-196c-45e9-89ff-fe14afe2f7ab"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"HTLV-I","subitem_subject_scheme":"Other"},{"subitem_subject":"Sjogren’s syndrome","subitem_subject_scheme":"Other"},{"subitem_subject":"cytokine","subitem_subject_scheme":"Other"},{"subitem_subject":"chemokine","subitem_subject_scheme":"Other"},{"subitem_subject":"in situ PCR","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Direct Infection of Primary Salivary Gland Epithelial Cells by Human T Lymphotropic Virus Type I in Patients With Sjogren's Syndrome","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Direct Infection of Primary Salivary Gland Epithelial Cells by Human T Lymphotropic Virus Type I in Patients With Sjogren's Syndrome"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-01"},"publish_date":"2016-05-01","publish_status":"0","recid":"4191","relation_version_is_last":true,"title":["Direct Infection of Primary Salivary Gland Epithelial Cells by Human T Lymphotropic Virus Type I in Patients With Sjogren's Syndrome"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:24:37.026929+00:00"}