@article{oai:nagasaki-u.repo.nii.ac.jp:00004211,
 author = {Nakane, Shunya and Higuchi, Osamu and Koga, Michiaki and Kanda, Takashi and Murata, Kenya and Suzuki, Takashi and Kurono, Hiroko and Kunimoto, Masanari and Kaida, Ken-ichi and Mukaino, Akihiro and Sakai, Waka and Maeda, Yasuhiro and Matsuo, Hidenori},
 issue = {3},
 journal = {PLOS ONE},
 month = {Mar},
 note = {Autoimmune autonomic ganglionopathy (AAG) is a rare acquired channelopathy that is characterized by pandysautonomia, in which autoantibodies to ganglionic nicotinic acetylcholine receptors (gAChR) may play a central role. Radioimmunoprecipitation (RIP) assays have been used for the sensitive detection of autoantibodies to gAChR in the serum of patients with AAG. Here, we developed luciferase immunoprecipitation systems (LIPS) to diagnose AAG based on IgGs to both the α3 and β4 gAChR subunits in patient serum. We reviewed the serological and clinical data of 50 Japanese patients who were diagnosed with AAG. With the LIPS testing, we detected anti-α3 and -β4 gAChR antibodies in 48% (24/50) of the patients. A gradual mode of onset was more common in the seropositive group than in the seronegative group. Patients with AAG frequently have orthostatic hypotension and upper and lower gastrointestinal tract symptoms, with or without anti-gAChR. The occurrence of autonomic symptoms was not significantly different between the seropositive and seronegative group, with the exception of achalasia in three patients from the seropositive group. In addition, we found a significant overrepresentation of autoimmune diseases in the seropositive group and endocrinological abnormalities as an occasional complication of AAG. Our results demonstrated that the LIPS assay was a useful novel tool for detecting autoantibodies against gAChR in patients with AAG., PLOS ONE, 10(3), e0118312; 2015},
 title = {Clinical Features of Autoimmune Autonomic Ganglionopathy and the Detection of Subunit-Specific Autoantibodies to the Ganglionic Acetylcholine Receptor in Japanese Patients},
 volume = {10},
 year = {2015}
}