@phdthesis{oai:nagasaki-u.repo.nii.ac.jp:00004268,
 author = {古川, 克郎},
 month = {Feb},
 note = {Keratinocyte growth factor (KGF) is considered to be one of the most important mitogens for lung epithelial cells. The objectives of this study were to confirm the effectiveness of intratracheal injection of recombinant human KGF (rhKGF) during compensatory lung growth and to optimize the instillation protocol. Here, trilobectomy in adult rat was performed, followed by intratracheal rhKGF instillation with low (0.4 mg/kg) and high (4 mg/kg) doses at various time-points. The proliferation of alveolar cells was assessed by the immunostaining for proliferating cell nuclear antigen (PCNA) in the residual lung. We also investigated other immunohistochemical parameters such as KGF, KGF receptor and surfactant protein A as well as terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Consequently, intratracheal single injection of rhKGF in high dose group significantly increased PCNA labeling index (LI) of alveolar cells in the remaining lung. Surprisingly, there was no difference in PCNA LI between low and high doses of rhKGF with daily injection, and PCNA LI reached a plateau level with 2 days-consecutive administration (about 60%). Our results indicate that even at low dose, daily intratracheal injection is effective to maintain high proliferative states during the early phase of compensatory lung growth., 長崎大学学位論文 学位記番号:博(医歯薬)甲第646号 学位授与年月日:平成26年2月5日, Author: Katsuro Furukawa, Keitaro Matsumoto, Takeshi Nagayasu, Tomomi Yamamoto-Fukuda, Shuichi Tobinaga, Takafumi Abo, Naoya Yamasaki, Tomoshi Tsuchiya, Takuro Miyazaki, Ryotaro Kamohara, Atsushi Nanashima, Masayuki Obatake, Takehiko Koji, Citation: ACTA HISTOCHEMICA ET CYTOCHEMICA, 46(6), pp.179-185; 2013, Nagasaki University (長崎大学), 博士(医学) (2014-02-05)},
 school = {Nagasaki University (長崎大学)},
 title = {Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat},
 year = {2014}
}