@phdthesis{oai:nagasaki-u.repo.nii.ac.jp:00000435,
 author = {BUNDI, MWEBIA MARTIN},
 month = {Dec},
 note = {Kenya is endemic for cholera with different waves of outbreaks having been documented since 1971. In recent years, new variants of Vibrio cholerae O1 have emerged and have replaced most of the traditional El Tor biotype globally. These strains also appear to have increased virulence, and it is important to describe and document their phenotypic and genotypic traits. This study characterized 146 V. cholerae O1 isolates from cholera outbreaks that occurred in Kenya between 1975 and 2017. Our study reports that the 1975?1984 strains had typical classical or El Tor biotype characters. New variants of V. cholerae O1 having traits of both classical and El Tor biotypes were observed from 2007 with all strains isolated between 2015 and 2017 being sensitive to polymyxin B and carrying both classical and El Tor type ctxB. All strains were resistant to Phage IV and harbored rstR, rtxC, hlyA, rtxA and tcpA genes specific for El Tor biotype indicating that the strains had an El Tor backbone. Pulsed field gel electrophoresis (PFGE) genotyping differentiated the isolates into 14 pulsotypes. The clustering also corresponded with the year of isolation signifying that the cholera outbreaks occurred as separate waves of different genetic fingerprints exhibiting different genotypic and phenotypic characteristics. The emergence and prevalence of V. cholerae O1 strains carrying El Tor type and classical type ctxB in Kenya are reported. These strains have replaced the typical El Tor biotype in Kenya and are potentially more virulent and easily transmitted within the population., 長崎大学学位論文 学位記番号:博(医歯薬)乙第57号 学位授与年月日:令和元年12月4日, Author: Martin Bundi, Mohammad Monir Shah, Eric Odoyo, Cyrus Kathiiko, Ernest Wandera, Gabriel Miring'u, Sora Guyo, Daniel Langat, Kouichi Morita,  Yoshio Ichinose, Citation: Microbiology and Immunology, 63(9), pp.350-358; 2019, Nagasaki University (長崎大学), 博士(医学) (2019-12-04)},
 school = {Nagasaki University (長崎大学)},
 title = {Characterization of Vibrio cholerae O1 isolates responsible for cholera outbreaks in Kenya between 1975 and 2017},
 year = {2019}
}