{"created":"2023-05-15T16:32:30.159917+00:00","id":4371,"links":{},"metadata":{"_buckets":{"deposit":"9030a03b-bde5-470a-9ce9-b15d45303ac2"},"_deposit":{"created_by":2,"id":"4371","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4371"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004371","sets":["25:26"]},"author_link":["17512","17510","17507","17508","17509","17511","17513"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"501","bibliographicPageStart":"493","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Exposure to ionizing radiation during childhood is a well-known risk factor for thyroid cancer. Our study evaluated the effect of age on the radiosensitivity of rat thyroid glands. Four-week-old (4W), 7 -week-old (7W), and 8-month-old (8M) male Wistar rats were exposed to 8 Gy of whole-body X-ray irradiation. Thyroids were removed 3-72 h after irradiation, and non-irradiated thyroids served as controls. Ki67-positivity and p53 binding protein 1 (53BP1) focus formation (a DNA damage response) were evaluated via immunohistochemistry. Amounts of proteins involved in DNA damage response (p53, p53 phosphorylated at serine 15, p21), apoptosis (cleaved caspase-3), and autophagy (LC3, p62) were determined via western blotting. mRNA levels of 84 key autophagy-related genes were quantified using polymerase chain reaction arrays. Ki67-positive cells in 4W (with high proliferative activity) and 7W thyroids significantly decreased in number post-irradiation. The number of 53BP1 foci and amount of p53 phosphorylated at serine 15 increased 3 h after irradiation, regardless of age. No increase in apoptosis or in the levels of p53, p21 or cleaved caspase-3 was detected for any ages. Levels of LC3-II and p62 increased in irradiated 4W but not 8M thyroids, whereas expression of several autophagy-related genes was higher in 4W than 8M irradiated thyroids. Irradiation increased the expression of genes encoding pro-apoptotic proteins in both 4W and 8M thyroids. In summary, no apoptosis or p53 accumulation was noted, despite the expression of some pro-apoptotic genes in immature and adult thyroids. Irradiation induced autophagy in immature, but not in adult, rat thyroids.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Radiation Research, 56(3), pp.493-501; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本放射線影響学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jrr/rrv003","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c The Author 2015. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology."},{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04493060","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13499157","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Japan Radiation Research Society"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsuu-Matsuyama, Mutsumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shichijo, Kazuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okaichi, Kumio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kurashige, Tomomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Hisayoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Shiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Masahiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"JRR56_493.pdf","filesize":[{"value":"703.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JRR56_493.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4371/files/JRR56_493.pdf"},"version_id":"30af07eb-fbd0-431d-b141-630ce3ed4b50"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Autophagy","subitem_subject_scheme":"Other"},{"subitem_subject":"p53 binding protein 1","subitem_subject_scheme":"Other"},{"subitem_subject":"Rat","subitem_subject_scheme":"Other"},{"subitem_subject":"Thyroid","subitem_subject_scheme":"Other"},{"subitem_subject":"X-ray","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of age on the sensitivity of the rat thyroid gland to ionizing radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of age on the sensitivity of the rat thyroid gland to ionizing radiation"}]},"item_type_id":"2","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-06-29"},"publish_date":"2015-06-29","publish_status":"0","recid":"4371","relation_version_is_last":true,"title":["Effect of age on the sensitivity of the rat thyroid gland to ionizing radiation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:22:18.351793+00:00"}