{"created":"2023-05-15T16:29:37.361057+00:00","id":441,"links":{},"metadata":{"_buckets":{"deposit":"71ef37fe-66d9-4bd3-ab28-0acdc0944327"},"_deposit":{"created_by":2,"id":"441","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"441"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000441","sets":["10:11"]},"author_link":["112147","112148","112146","112145","112144"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-11-29","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"104625","bibliographicVolumeNumber":"110","bibliographic_titles":[{"bibliographic_title":"Archives of Oral Biology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Accumulating evidence suggests an association between periodontitis and several systemic diseases, such as atherosclerosis. In the lesions of these diseases, nucleotide-binding domain leucine-rich repeat-containing protein 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) and caspase-1 form inflammasome complex, which leads to the functional maturation of interleukin (IL)-1β via cleavage of caspase-1 in macrophages. IL-1β plays a critical role in the etiology of these diseases; however, inflammasome priming?specifically, IL-1β and NLRP3 upregulation?is necessary for effective IL-1β production. We investigated the effect of initial periodontal treatment on the inflammasome priming of peripheral blood mononuclear cells (PBMCs). Methods: Twenty-two patients with chronic periodontitis were enrolled in this study and given initial periodontal treatment. Peripheral blood samples were collected at baseline and re-evaluation (41.1 ± 29.1 d after the treatment), and the relative expression of IL-1β, and three inflammasome components, ASC, NLRP3 and Caspase-1, mRNA was determined using quantitative reverse transcription PCR. PBMCs were stimulated with silica crystals, and the IL-1β secretion was measured via enzyme-linked immunosorbent assay. Results: Probing pocket depth and bleeding on probing (BOP) were significantly improved after the treatment. Expression of IL-1β and ASC in the PBMCs decreased after the treatment. PBMCs stimulated with silica crystals secreted IL-1β. The treatment attenuated IL-1β secretion by PBMCs in low BOP percentages group whereas IL-1β secretion was increased in high BOP percentages group. Conclusion: Periodontal treatment altered the inflammasome priming status of the PBMCs, however, the effects on systemic diseases need to be further investigated.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Archives of Oral Biology, 110, art.no.104625; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Ltd."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.archoralbio.2019.104625","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2019 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00039969","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Higuchi, Kanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SM, Ziauddin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Yasunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ozaki, Yukio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimura, Atsutoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-25"}],"displaytype":"detail","filename":"AOB110_104625.pdf","filesize":[{"value":"870.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AOB110_104625.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/441/files/AOB110_104625.pdf"},"version_id":"25cac6a2-459c-4ea2-903d-2b970101ddfd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chronic periodontitis","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammation","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammasome","subitem_subject_scheme":"Other"},{"subitem_subject":"Interleukin-1β","subitem_subject_scheme":"Other"},{"subitem_subject":"Mononuclear leukocyte","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Initial periodontal treatment affects nucleotide-binding domain leucine-rich repeat-containing protein 3 inflammasome priming in peripheral blood mononuclear cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Initial periodontal treatment affects nucleotide-binding domain leucine-rich repeat-containing protein 3 inflammasome priming in peripheral blood mononuclear cells"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-29"},"publish_date":"2020-11-29","publish_status":"0","recid":"441","relation_version_is_last":true,"title":["Initial periodontal treatment affects nucleotide-binding domain leucine-rich repeat-containing protein 3 inflammasome priming in peripheral blood mononuclear cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-15T23:01:33.326449+00:00"}