{"created":"2023-05-15T16:32:35.331580+00:00","id":4491,"links":{},"metadata":{"_buckets":{"deposit":"84ecc4ea-ef09-48ab-a46c-57905f3d282a"},"_deposit":{"created_by":2,"id":"4491","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4491"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004491","sets":["73:74"]},"author_link":["17931","17932","17933","17934","17929","17930"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"435","bibliographicPageStart":"427","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"Journal of Drug Targeting"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Achieving long-term gene expression in kidney will be beneficial for gene therapy of renal and congenital diseases, genetic studies constructing animal disease models, and the functional analysis of disease-related genes. Purpose: The purpose of this study was to develop an in vivo long-term gene expression system in murine kidney using φC31 integrase. Methods: Gene expression in cultured RENCA, TCMK-1, and HEK293 cells was assessed. The long-term in vivo gene expression system in the kidney was achieved by co-transfecting 5 μg of pORF-luc/attB as a donor plasmid and 20 μg of pCMV-luc as a helper plasmid into the right kidney of mice by electroporation. Luciferase expression levels were measured to determine longevity of the expression. Results: Significantly high luciferase expression levels were observed in cultured RENCA, TCMK-1, and HEK293 cells over 1 month compared with controls (non-integrase system). The luciferase cDNA sequence was integrated at a pseudo attP site termed mpsL1. In vivo luciferase expression levels in the integrase group were sustained and significantly higher than those in the control group over 2 months. Furthermore, φC31 integrase-transfected cells had less genomic DNA damage caused by integrase expression. Discussion and conclusion: These results demonstrated that the φC31 integrase system could produce long-term (2 months) in vivo gene expression in mouse kidney.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Drug Targeting, 23(5), pp.427-435; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Informa Healthcare"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3109/1061186X.2014.1002788","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2015 Informa UK Ltd."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1061186X","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"10292330","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Otani, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukai, Hidefumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuchigami, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Fumiyoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashida, Mitsuru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"JDT23_427.pdf","filesize":[{"value":"651.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JDT23_427.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4491/files/JDT23_427.pdf"},"version_id":"b1f7f23c-aa26-44a8-abda-5f5f6b7f93e5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Electroporation","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene therapy","subitem_subject_scheme":"Other"},{"subitem_subject":"in vivo long-term gene expression","subitem_subject_scheme":"Other"},{"subitem_subject":"Kidney","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmid DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"Transfection","subitem_subject_scheme":"Other"},{"subitem_subject":"φC31 integrase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Long-term in vivo gene expression in mouse kidney using φC31 integrase and electroporation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Long-term in vivo gene expression in mouse kidney using φC31 integrase and electroporation"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-06-02"},"publish_date":"2016-06-02","publish_status":"0","recid":"4491","relation_version_is_last":true,"title":["Long-term in vivo gene expression in mouse kidney using φC31 integrase and electroporation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:20:53.318751+00:00"}