{"created":"2023-05-15T16:29:38.220003+00:00","id":461,"links":{},"metadata":{"_buckets":{"deposit":"3f7a9bf3-a4d8-446b-a6a5-cc625367c727"},"_deposit":{"created_by":2,"id":"461","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"461"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000461","sets":["10:11"]},"author_link":["2402","2399","2401","2398","2400"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"642","bibliographicPageStart":"629","bibliographicVolumeNumber":"401","bibliographic_titles":[{"bibliographic_title":"Biological Chemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Prevotella intermedia, a gram-negative anaerobic rod, is frequently observed in subgingival polymicrobial biofilm from adults with chronic periodontitis. Peptidases in periodontopathic bacteria are considered to function as etiological reagents. Pre. intermedia OMA14 cells abundantly express an unidentified cysteine peptidase specific for Arg-4-methycoumaryl-7- amide (MCA). BAU17746 (locus tag, PIOMA14_I_1238) and BAU18827 (locus tag, PIOMA14_II_0322) emerged as candidates of this peptidase from the substrate specificity and sequence similarity with C69-family Streptococcus gordonii Arg-aminopeptidase. The recombinant form of the former solely exhibited hydrolyzing activity toward Arg-MCA, and BAU17746 possesses a 26.6% amino acid identity with the C69-family Lactobacillus helveticus dipeptidase A. It was found that BAU17746 as well as L. helveticus dipeptidase A was a P1-position Arg-specific dipeptidase A, although the L. helveticus entity, a representative of the C69 family, had been reported to be specific for Leu and Phe. The fulllength form of BAU17746 was intramolecularly processed to a mature form carrying the N-terminus of Cys15. In conclusion, the marked Arg-MCA-hydrolyzing activity in Pre. intermedia was mediated by BAU17746 belonging to the C69-family dipeptidase A, in which the mature form carries an essential cysteine at the N-terminus.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological Chemistry, 401(5), pp.629-642; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Walter de Gruyter"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1515/hsz-2019-0387","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 by Walter de Gruyter Berlin/Boston."},{"subitem_rights":"The final publication is available at www.degruyter.com"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14316730","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14374315","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sarwar, Mohammad Tanvir"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohara-Nemoto, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayakawa, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naito, Mariko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nemoto, Takayuki K."}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"BiolChem_Sarwar2020.pdf","filesize":[{"value":"3.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BiolChem_Sarwar2020.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/461/files/BiolChem_Sarwar2020.pdf"},"version_id":"930567bc-8f8e-41cf-9a73-67e762733662"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"dipeptidase A","subitem_subject_scheme":"Other"},{"subitem_subject":"cysteine peptidase","subitem_subject_scheme":"Other"},{"subitem_subject":"autoprocessing","subitem_subject_scheme":"Other"},{"subitem_subject":"Prevotella intermedia","subitem_subject_scheme":"Other"},{"subitem_subject":"substrate specificity","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontal disease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-12-18"},"publish_date":"2020-12-18","publish_status":"0","recid":"461","relation_version_is_last":true,"title":["Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-05-15T20:21:10.377981+00:00"}