@article{oai:nagasaki-u.repo.nii.ac.jp:00004665,
 author = {Kitajima, Yuriko and Doi, Hanako and Ono, Yusuke and Urata, Yoshishige and Goto, Shinji and Kitajima, Michio and Miura, Kiyonori and Li, Tao-Sheng and Masuzaki, Hideaki},
 journal = {Scientific Reports},
 month = {Aug},
 note = {Postmenopausal disorders are frequently observed in various organs, but their relationship with estrogen deficiency and mechanisms remain unclear. As tissue-specific stem cells have been found to express estrogen receptors, we examined the hypothesis that estrogen deficiency impairs stem cells, which consequently contributes to postmenopausal disorders. Six-week-old C57BL/6 female mice were ovariectomized, following which they received 17β-estradiol replacement or vehicle (control). Sham-operated mice were used as healthy controls. All mice were killed for evaluation 2 months after treatments. Compared with the healthy control, ovariectomy significantly decreased uterine weight, which was partially recovered by 17β-estradiol replacement. Ovariectomy significantly increased the numbers of c-kit-positive hematopoietic stem/progenitor cells in bone marrow, but impaired their capacity to grow mixed cell-type colonies in vitro. Estrogen replacement further increased the numbers of c-kit-positive hematopoietic stem/progenitor cells in bone marrow, without significantly affecting colony growth in vitro. The number of CD105-positive mesenchymal stem cells in bone marrow also significantly decreased after ovariectomy, but completely recovered following estrogen replacement. Otherwise, neither ovariectomy nor estrogen replacement changed the number of Pax7-positive satellite cells, which are a skeletal muscle-type stem cell. Estrogen deficiency heterogeneously affected tissue-specific stem cells, suggesting a likely and direct relationship with postmenopausal disorders., Scientific Reports, 5, 12861; 2015},
 title = {Estrogen deficiency heterogeneously affects tissue specific stem cells in mice},
 volume = {5},
 year = {2015}
}