{"created":"2023-05-15T16:32:42.839632+00:00","id":4665,"links":{},"metadata":{"_buckets":{"deposit":"b54737e8-6966-4369-8d73-ca8548b6b135"},"_deposit":{"created_by":2,"id":"4665","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4665"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004665","sets":["25:26"]},"author_link":["18751","18752","18753","18745","18747","18750","18749","18748","18746"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08-06","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"12861","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Postmenopausal disorders are frequently observed in various organs, but their relationship with estrogen deficiency and mechanisms remain unclear. As tissue-specific stem cells have been found to express estrogen receptors, we examined the hypothesis that estrogen deficiency impairs stem cells, which consequently contributes to postmenopausal disorders. Six-week-old C57BL/6 female mice were ovariectomized, following which they received 17β-estradiol replacement or vehicle (control). Sham-operated mice were used as healthy controls. All mice were killed for evaluation 2 months after treatments. Compared with the healthy control, ovariectomy significantly decreased uterine weight, which was partially recovered by 17β-estradiol replacement. Ovariectomy significantly increased the numbers of c-kit-positive hematopoietic stem/progenitor cells in bone marrow, but impaired their capacity to grow mixed cell-type colonies in vitro. Estrogen replacement further increased the numbers of c-kit-positive hematopoietic stem/progenitor cells in bone marrow, without significantly affecting colony growth in vitro. The number of CD105-positive mesenchymal stem cells in bone marrow also significantly decreased after ovariectomy, but completely recovered following estrogen replacement. Otherwise, neither ovariectomy nor estrogen replacement changed the number of Pax7-positive satellite cells, which are a skeletal muscle-type stem cell. Estrogen deficiency heterogeneously affected tissue-specific stem cells, suggesting a likely and direct relationship with postmenopausal disorders.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Scientific Reports, 5, 12861; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/srep12861","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kitajima, Yuriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Doi, Hanako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ono, Yusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urata, Yoshishige"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Shinji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitajima, Michio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Kiyonori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Li, Tao-Sheng"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuzaki, Hideaki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"SciRep5_12861.pdf","filesize":[{"value":"651.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SciRep5_12861.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4665/files/SciRep5_12861.pdf"},"version_id":"eaa5914e-0775-4c64-8c90-c5429e83ad4c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Estrogen deficiency heterogeneously affects tissue specific stem cells in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Estrogen deficiency heterogeneously affects tissue specific stem cells in mice"}]},"item_type_id":"2","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-09-03"},"publish_date":"2015-09-03","publish_status":"0","recid":"4665","relation_version_is_last":true,"title":["Estrogen deficiency heterogeneously affects tissue specific stem cells in mice"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:17:46.415869+00:00"}