{"created":"2023-05-15T16:32:45.629165+00:00","id":4727,"links":{},"metadata":{"_buckets":{"deposit":"8e728a1b-70d6-4a8b-849c-da5278b816fe"},"_deposit":{"created_by":2,"id":"4727","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4727"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004727","sets":["35:36"]},"author_link":["19199","19207","19202","19198","19205","19196","19206","19204","19201","19197","19203","19200"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageStart":"e0004021","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLOS Neglected Tropical Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Both Schistosoma mansoni and Schistosoma haematobium cause schistosomiasis in sub-Saharan Africa. We assessed the diagnostic value of selected Schistosoma antigens for the development of a multiplex serological immunoassay for sero-epidemiological surveillance. Methodology/Principal Findings: Diagnostic ability of recombinant antigens from S. mansoni and S. haematobium was assessed by Luminex multiplex immunoassay using plasma from school children in two areas of Kenya, endemic for different species of schistosomiasis. S. mansoni serine protease inhibitor (SERPIN) and Sm-RP26 showed significantly higher reactivity to patient plasma as compared to the control group. Sm-Filamin, Sm-GAPDH, Sm-GST, Sm-LAP1, Sm-LAP2, Sm-Sm31, Sm-Sm32 and Sm-Tropomyosin did not show difference in reactivity between S. mansoni infected and uninfected pupils. Sm-RP26 was cross-reactive to plasma from S. haematobium patients, whereas Sm-SERPIN was species-specific. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. ROC analysis for Sm-RP26, Sm-SERPIN and Sh-SERPIN showed AUC values of 0.833, 0.888 and 0.947, respectively. Using Spearman’s rank correlation coefficient analysis, we also found significant positive correlation between the number of excreted eggs and median fluorescence intensity (MFI) from the multiplex immunoassays for Sm-SERPIN (ρ = 0.430, p-value = 0.003) and Sh-SERPIN (ρ = 0.433, p-value = 0.006). Conclusions/Significance: Sm-SERPIN is a promising species-specific diagnostic antigen. Sh-SEPRIN was partially cross-reactive to S. mansoni infected patients. SERPINs showed correlation with the number of excreted eggs. These indicate prospects for inclusion of SERPINs in the multiplex serological immunoassay system.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLOS Neglected Tropical Diseases, 9(8), e0004021; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pntd.0004021","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2015 Tanigawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19352735","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanigawa, Chihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujii, Yoshito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miura, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nzou, Samson Muuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mwangi, Anne Wanjiru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagi, Sachiyo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hamano, Shinjiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Njenga, Sammy M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mbanefo, Evaristus Chibunna"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirayama, Kenji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mwau, Matilu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Satoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoSNeg9_4021.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoSNeg9_4021.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4727/files/PLoSNeg9_4021.pdf"},"version_id":"9a80a5ef-9de8-4f07-a831-4483894701a0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-10-01"},"publish_date":"2015-10-01","publish_status":"0","recid":"4727","relation_version_is_last":true,"title":["Species-Specific Serological Detection for Schistosomiasis by Serine Protease Inhibitor (SERPIN) in Multiplex Assay"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:16:31.024064+00:00"}