{"created":"2023-05-15T16:32:46.976021+00:00","id":4758,"links":{},"metadata":{"_buckets":{"deposit":"a59c2e68-311e-4cc0-b936-dd23660640d5"},"_deposit":{"created_by":2,"id":"4758","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4758"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004758","sets":["35:36"]},"author_link":["19436","19434","19439","19440","19438","19435","19437"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-09-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"414","bibliographicPageStart":"410","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"Japanese Journal of Infectious Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chikungunya (CHIK) is a mosquito-borne viral disease. In the 2004 CHIK outbreak in Kenya, diagnosis was delayed because of the lack of accurate diagnostics. Therefore, this study aimed to develop and evaluate an in-house IgM-capture enzyme linked immunosorbent assay (ELISA) (in-house ELISA) for the detection of chikungunya virus (CHIKV) infections. Anti-CHIKV antibodies were raised in rabbits, purified and conjugated to horseradish peroxidase. These anti-CHIKV antibodies and cell-culture derived antigen were used to develop the ELISA. To validate the in-house ELISA, 148 patient sera from the 2005 Comoros CHIK outbreak were tested with centers for disease control and prevention (CDC) IgM-capture ELISA (CDC ELISA) and focus reduction neutralization test (FRNT) as reference assays. The in-house ELISA had a sensitivity of 97.6% and specificity of 81.3% compared to the CDC ELISA and a sensitivity of 91.1% and specificity of 96.7% compared to FRNT. Furthermore, 254 clinically suspected dengue patient samples from Eastern Kenya, collected in 2013, were tested for CHIKV IgM using the in-house ELISA. Out of the 254 samples, 26 (10.2%) were IgM positive, and of these 26 samples, 17 were further analyzed by FRNT and 14 (82.4%) were positive. The in-house ELISA was able to diagnose CHIKV infection among suspected dengue cases in the 2013 outbreak.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Japanese Journal of Infectious Diseases, 68(5), pp.410-414; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立感染症研究所"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.7883/yoken.JJID.2014.482","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2015 National Institute of Infectious Diseases"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13446304","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18842836","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"National Institute of Infectious Diseases"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wasonga, Caroline"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Shingo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimotho, James"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Kouichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ongus, Juliette"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sang, Rosemary"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Musila, Lillian"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"JID68_410.pdf","filesize":[{"value":"333.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JID68_410.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4758/files/JID68_410.pdf"},"version_id":"23d9ba83-4064-4040-8271-e0ecf6105e50"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chikungunya virus","subitem_subject_scheme":"Other"},{"subitem_subject":"Focus reduction neutralization test","subitem_subject_scheme":"Other"},{"subitem_subject":"IgM-capture ELISA","subitem_subject_scheme":"Other"},{"subitem_subject":"Kenya","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-10-21"},"publish_date":"2015-10-21","publish_status":"0","recid":"4758","relation_version_is_last":true,"title":["Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:15:50.751882+00:00"}