@article{oai:nagasaki-u.repo.nii.ac.jp:00000492,
 author = {Karbwang, Juntra and Na-Bangchang, Kesara and Narahara, Chisato and Saeheng, Teerachat and Chaijaroenkul, Wanna and Dumre, ShyamPrakash},
 issue = {1},
 journal = {Journal of Research in Medical Sciences},
 month = {Jan},
 note = {Background: Cholangiocarcinoma (CCA) is a neglected disease prevalent in developing countries with high burden and mortality rate, and there is no effective treatment. We aimed to investigate β-eudesmol molecular target of action in human CCA cell lines using the selected key molecules of apoptotic pathways. Materials and Methods: Two CCA cell lines (HuH28 and HuCCT1) were assessed at different time points after β-eudesmol treatment for mRNA and protein expression profiles of caspase-3, -8, -9, p53, p21, Bcl-2, and Bax by real-time polymerase chain reaction and western blot, respectively. Results: β-eudesmol induced expressions of p21 and p53 in mRNA/protein level in HuH28 and HuCCT1 cells. These CCA cells also expressed caspase-3, -8, -9 and bax (mRNA and/or protein level) among others after β-eudesmol treatment indicating its role in both intrinsic and extrinsic caspase-dependent apoptotic pathways. Conclusion: The study demonstrated that β-eudesmol induced the expression of apoptosis pathway proteins, suggesting its potential role in promoting the caspase-dependent apoptotic pathway, and induction of the cell cycle arrest in CCA cell lines. β-eudesmol can be considered as a potential compound for further investigation as an anti-CCA agent., Journal of Research in Medical Sciences, 25(1), art.no.7; 2020},
 title = {β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines},
 volume = {25},
 year = {2020}
}