{"created":"2023-05-15T16:29:39.562080+00:00","id":492,"links":{},"metadata":{"_buckets":{"deposit":"fedb8d56-4e71-4b0b-b70e-9d14d6e26136"},"_deposit":{"created_by":2,"id":"492","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"492"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000492","sets":["35:36"]},"author_link":["2627","2624","2625","2628","2626","2623"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"7","bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"Journal of Research in Medical Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Cholangiocarcinoma (CCA) is a neglected disease prevalent in developing countries with high burden and mortality rate, and there is no effective treatment. We aimed to investigate β-eudesmol molecular target of action in human CCA cell lines using the selected key molecules of apoptotic pathways. Materials and Methods: Two CCA cell lines (HuH28 and HuCCT1) were assessed at different time points after β-eudesmol treatment for mRNA and protein expression profiles of caspase-3, -8, -9, p53, p21, Bcl-2, and Bax by real-time polymerase chain reaction and western blot, respectively. Results: β-eudesmol induced expressions of p21 and p53 in mRNA/protein level in HuH28 and HuCCT1 cells. These CCA cells also expressed caspase-3, -8, -9 and bax (mRNA and/or protein level) among others after β-eudesmol treatment indicating its role in both intrinsic and extrinsic caspase-dependent apoptotic pathways. Conclusion: The study demonstrated that β-eudesmol induced the expression of apoptosis pathway proteins, suggesting its potential role in promoting the caspase-dependent apoptotic pathway, and induction of the cell cycle arrest in CCA cell lines. β-eudesmol can be considered as a potential compound for further investigation as an anti-CCA agent.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Research in Medical Sciences, 25(1), art.no.7; 2020","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wolters Kluwer Medknow Publications"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.4103/jrms.JRMS_291_19","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 Journal of Research in Medical Sciences. This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"17351995","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Karbwang, Juntra"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Na-Bangchang, Kesara"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Narahara, Chisato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saeheng, Teerachat"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chaijaroenkul, Wanna"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dumre, ShyamPrakash"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"JRMS25_7.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JRMS25_7.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/492/files/JRMS25_7.pdf"},"version_id":"d4d674de-2773-4505-b515-afac2f51d926"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"cholangiocarcinoma","subitem_subject_scheme":"Other"},{"subitem_subject":"mode of action","subitem_subject_scheme":"Other"},{"subitem_subject":"molecular targets","subitem_subject_scheme":"Other"},{"subitem_subject":"β?eudesmol","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-02-05"},"publish_date":"2020-02-05","publish_status":"0","recid":"492","relation_version_is_last":true,"title":["β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:15:50.410780+00:00"}