{"created":"2023-05-15T16:32:55.454414+00:00","id":4957,"links":{},"metadata":{"_buckets":{"deposit":"fc826ef2-97fb-45e2-8723-66ce92ff088f"},"_deposit":{"created_by":2,"id":"4957","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"4957"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00004957","sets":["29:30"]},"author_link":["20328","20330","20325","20327","20329","20326","20324","20323"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-09-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageStart":"e0137958","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"PLOS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Prion diseases are neurodegenerative disorders caused by the accumulation of abnormal prion protein (PrPSc) in the central nervous system. With the aim of elucidating the mechanism underlying the accumulation and degradation of PrPSc, we investigated the role of autophagy in its degradation, using cultured cells stably infected with distinct prion strains. The effects of pharmacological compounds that inhibit or stimulate the cellular signal transduction pathways that mediate autophagy during PrPSc degradation were evaluated. The accumulation of PrPSc in cells persistently infected with the prion strain Fukuoka-1 (FK), derived from a patient with Gerstmann-Straussler-Scheinker syndrome, was significantly increased in cultures treated with the macroautophagy inhibitor 3-methyladenine (3MA) but substantially reduced in those treated with the macroautophagy inducer rapamycin. The decrease in FK-derived PrPSc levels was mediated, at least in part, by the phosphatidylinositol 3-kinase/MEK signalling pathway. By contrast, neither rapamycin nor 3MA had any apparently effect on PrPSc from either the 22L or the Chandler strain, indicating that the degradation of PrPSc in host cells might be strain-dependent.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLOS ONE, 10(9), e0137958; 2015","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0137958","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2015 Ishibashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishibashi, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Homma, Takujiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagaki, Takehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuse, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sano, Kazunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takatsuki, Hanae"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Atarashi, Ryuichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishida, Noriyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoS10_137958.pdf","filesize":[{"value":"4.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS10_137958.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/4957/files/PLoS10_137958.pdf"},"version_id":"230f9805-48ff-4b57-ab14-0ba48abb0523"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Strain-Dependent Effect of Macroautophagy on Abnormally Folded Prion Protein Degradation in Infected Neuronal Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Strain-Dependent Effect of Macroautophagy on Abnormally Folded Prion Protein Degradation in Infected Neuronal Cells"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-01-07"},"publish_date":"2016-01-07","publish_status":"0","recid":"4957","relation_version_is_last":true,"title":["Strain-Dependent Effect of Macroautophagy on Abnormally Folded Prion Protein Degradation in Infected Neuronal Cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:13:56.868182+00:00"}