{"created":"2023-05-15T16:32:58.557175+00:00","id":5029,"links":{},"metadata":{"_buckets":{"deposit":"913cb9fa-c9c8-4164-8d8a-7318b280eb50"},"_deposit":{"created_by":2,"id":"5029","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5029"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005029","sets":["29:30:1713167819629:311"]},"author_link":["20692","20689","20696","20695","20693","20690","20691","20694"],"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"76","bibliographicPageStart":"69","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"Acta medica Nagasakiensia"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background:　When multipass transmembrane molecules are located on the cell surface, there may be interaction with not\nonly bioactive molecules but also pathogenic molecules in areas protruding outside the cell. In antibody-mediated autoimmune\ndisorders, it has been found that the autoantibodies occasionally attack membrane molecules on the cell surface, thus causing\nthe disease such as myasthenia gravis. In such cases, highly sensitive autoantibody detection technology is required for early\ndiagnosis. However, autoantibody analysis technology that is specialized for membrane molecules is still under development.\nHere we demonstrate a novel method for detecting of antibodies against the extracellular portions of multipass transmembrane\nmolecules.\nMethods:　Antibodies for muscarinic acetylcholine receptor type3 (M3R) were detected with two kinds of luciferase immunoprecipitaion\nsystems (LIPS), conventional LIPS (cLIPS) and its modified application, termed modified LIPS (mLIPS). In mLIPS, antibodies\nagainst extracellular portions of membrane molecules could be preferentially detected.\nResults:　An antibody to the amino-terminal portion of human M3R was detected with modified LIPS with a high sensitivity. In\ncontrast, an antibody to the carboxyl-terminal portion was not detected with mLIPS, because it did not interact with intracellular\nportions of M3R in living cells. We also found antibodies for M3R in a patient serum with Sjogren’s syndrome.\nConclusion:　Our technology has a promising future, and we hope that it will be applied in the analysis of antibodies against a\ndiverse range of multipass transmembrane molecules, including GPCRs.","subitem_description_type":"Abstract"}]},"item_3_description_64":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Acta medica Nagasakiensia, 60(2), pp.69-76; 2016","subitem_description_type":"Other"}]},"item_3_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nagasaki University School of Medicine"}]},"item_3_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00508430","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00016055","subitem_source_identifier_type":"ISSN"}]},"item_3_text_62":{"attribute_name":"sortkey","attribute_value_mlt":[{"subitem_text_value":"6"}]},"item_3_text_63":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"長崎大学医学部"}]},"item_3_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mukaino, Akihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higuchi, Osamu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakane, Shunya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maeda, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakai, Waka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuo, Hidenori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Atsushi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"ActMed60_69.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ActMed60_69.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5029/files/ActMed60_69.pdf"},"version_id":"892ec27c-ebc7-44a9-8772-bb1f7430fd24"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GPCR","subitem_subject_scheme":"Other"},{"subitem_subject":"LIPS","subitem_subject_scheme":"Other"},{"subitem_subject":"AChR","subitem_subject_scheme":"Other"},{"subitem_subject":"luciferase","subitem_subject_scheme":"Other"},{"subitem_subject":"autoantibody","subitem_subject_scheme":"Other"},{"subitem_subject":"autoimmune disease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A modified application of the luciferase immunoprecipitation systems for detecting antibodies to the G protein-coupled receptors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A modified application of the luciferase immunoprecipitation systems for detecting antibodies to the G protein-coupled receptors"}]},"item_type_id":"3","owner":"2","path":["311"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-10"},"publish_date":"2016-02-10","publish_status":"0","recid":"5029","relation_version_is_last":true,"title":["A modified application of the luciferase immunoprecipitation systems for detecting antibodies to the G protein-coupled receptors"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2024-04-16T05:28:11.497911+00:00"}