{"created":"2023-05-15T16:33:02.864784+00:00","id":5129,"links":{},"metadata":{"_buckets":{"deposit":"534c4cac-a9e6-4d8b-aa46-2c4295922b36"},"_deposit":{"created_by":2,"id":"5129","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5129"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005129","sets":["29:30"]},"author_link":["21187","21183","21186","21188","21184","21190","21185","21191","21189","21192"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"1480","bibliographicPageStart":"1467","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"International Journal of Oncology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Epigenetic parameters such as DNA methylation and histone modifications play pivotal roles in carcinogenesis. Global histone modification patterns have been implicated as possible predictors of cancer recurrence and prognoses in a great variety of tumor entities. Our study was designed to evaluate the association among trimethylated histone H3 at lysine 27 (H3K27me3), clinicopathological variables and outcome in early-stage non-small cell lung cancer (NSCLC). The expression of H3K27me3 and its methyltransferase, enhancer of zeste homolog 2 (EZH2) together with proliferating cell nuclear antigen (PCNA) were evaluated by immunohistochemistry in normal lung tissue (n=5) and resected NSCLC patients (n=42). In addition, the specificity of antibody for H3K27me3 was tested by western blot analysis. The optimal cut-off point of H3K27me3 expression for prognosis was determined by the X-tile program. The prognostic significance was determined by means of Kaplan-Meier survival estimates and log-rank tests. As a result, enhanced trimethylation of H3K27me3 was correlated with longer overall survival (OS) and better prognosis (P<0.05). Moreover, both univariate and multivariate analyses indicated that H3K27me3 level was a significant and independent predictor of better survival (hazard ratio, 0.187; 95% confidence interval, 0.066-0.531, P=0.002). Furthermore, H3K27me3 expression was positively correlated with DNA methylation level at CCGG sites while reversely related to EZH2 expression (P<0.05). In conclusion, H3K27me3 level defines unrecognized subgroups of NSCLC patients with distinct epigenetic phenotype and clinical outcome, and can probably be used as a novel predictor for better prognosis in NSCLC patients.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Oncology, 43(5), pp.1467-1480; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Spandidos Publications"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3892/ijo.2013.2062","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c Spandidos Publications 2013. All rights reserved."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10196439","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"17912423","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chen, Xiaohui"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Song, Ning"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumoto, Keitaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nanashima, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagayasu, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Tomayoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ying, Mingang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Endo, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wu, Zhiren"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koji, Takehiko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"IJO43_1467.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJO43_1467.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5129/files/IJO43_1467.pdf"},"version_id":"b72ec4ba-b759-4af6-8f48-c60d308b0a1c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA methylation","subitem_subject_scheme":"Other"},{"subitem_subject":"Enhancer of zeste homolog 2","subitem_subject_scheme":"Other"},{"subitem_subject":"Epigenetics","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-small cell lung cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"Trimethylated histone H3 at lysine 27","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"High expression of trimethylated histone H3 at lysine 27 predicts better prognosis in non-small cell lung cancer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High expression of trimethylated histone H3 at lysine 27 predicts better prognosis in non-small cell lung cancer"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-01"},"publish_date":"2014-06-01","publish_status":"0","recid":"5129","relation_version_is_last":true,"title":["High expression of trimethylated histone H3 at lysine 27 predicts better prognosis in non-small cell lung cancer"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:11:35.667997+00:00"}