@article{oai:nagasaki-u.repo.nii.ac.jp:00005140,
 author = {Kato, Takuma and Tanaka, Masakazu and Oba, Makoto},
 issue = {12},
 journal = {PLoS ONE},
 month = {Dec},
 note = {Several protein transfection reagents are commercially available and are powerful tools for elucidating function of a protein in a cell. Here we described protein transfection studies of the commercially available reagents, Pro- DeliverIN, Xfect, and TuboFect, using Huh-7 multicellular tumor spheroid (MCTS) as a three-dimensional in vitro tumor model. A cellular uptake study using specific endocytosis inhibitors revealed that each reagent was internalized into Huh-7 MCTS by different mechanisms, which were the same as monolayer cultured Huh-7 cells. A certain amount of Pro-DeliverIN and Xfect was uptaken by Huh-7 cells through caveolae-mediated endocytosis, which may lead to transcytosis through the surface-first layered cells of MCTS. The results presented here will help in the choice and use of protein transfection reagents for evaluating anti-tumor therapeutic proteins against MCTS models., PLoS ONE, 8(12), e82876; 2013},
 title = {Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells},
 volume = {8},
 year = {2013}
}