{"created":"2023-05-15T16:33:03.342226+00:00","id":5140,"links":{},"metadata":{"_buckets":{"deposit":"368eec8b-e3f8-4cfc-af0f-b195c7309919"},"_deposit":{"created_by":2,"id":"5140","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5140"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005140","sets":["73:74"]},"author_link":["21270","21271","21269"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-12-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageStart":"e82876","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Several protein transfection reagents are commercially available and are powerful tools for elucidating function of a protein in a cell. Here we described protein transfection studies of the commercially available reagents, Pro- DeliverIN, Xfect, and TuboFect, using Huh-7 multicellular tumor spheroid (MCTS) as a three-dimensional in vitro tumor model. A cellular uptake study using specific endocytosis inhibitors revealed that each reagent was internalized into Huh-7 MCTS by different mechanisms, which were the same as monolayer cultured Huh-7 cells. A certain amount of Pro-DeliverIN and Xfect was uptaken by Huh-7 cells through caveolae-mediated endocytosis, which may lead to transcytosis through the surface-first layered cells of MCTS. The results presented here will help in the choice and use of protein transfection reagents for evaluating anti-tumor therapeutic proteins against MCTS models.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 8(12), e82876; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0082876","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2013 Kato et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kato, Takuma"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Masakazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oba, Makoto"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoS8_82876.pdf","filesize":[{"value":"752.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS8_82876.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5140/files/PLoS8_82876.pdf"},"version_id":"4e24efd8-5d56-46ad-9f06-0698ebc137dd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-03-05"},"publish_date":"2014-03-05","publish_status":"0","recid":"5140","relation_version_is_last":true,"title":["Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:11:19.607760+00:00"}