{"created":"2023-05-15T16:33:06.759543+00:00","id":5219,"links":{},"metadata":{"_buckets":{"deposit":"b1f792a8-17eb-4d64-9476-149282f92f77"},"_deposit":{"created_by":2,"id":"5219","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5219"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005219","sets":["10:11"]},"author_link":["21787","21784","21781","21785","21780","21786","21788","21782","21783"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"54","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"BMC Infectious Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: The rapid identification of acid-fast bacilli recovered from patient specimens as Mycobacterium tuberculosis complex (MTC) is critically important for accurate diagnosis and treatment. A thin-layer immunochromatographic (TLC) assay using anti-MPB64 or anti-MPT64 monoclonal antibodies was developed to discriminate between MTC and non-tuberculosis mycobacteria (NTM). Capilia TB-Neo, which is the improved version of Capilia TB, is recently developed and needs to be evaluated.Methods: Capilia TB-Neo was evaluated by using reference strains including 96 Mycobacterium species (4 MTC and 92 NTM) and 3 other bacterial genera, and clinical isolates (500 MTC and 90 NTM isolates). M. tuberculosis isolates tested negative by Capilia TB-Neo were sequenced for mpt64 gene.Results: Capilia TB-Neo showed 100% agreement to a subset of reference strains. Non-specific reaction to M. marinum was not observed. The sensitivity and specificity of Capilia TB-Neo to the clinical isolates were 99.4% (99.6% for M. tuberculosis, excluding M. bovis BCG) for clinical MTC isolates and 100% for NTM isolates tested, respectively. Two M. tuberculosis isolates tested negative by Capilia TB-Neo: one harbored a 63-bp deletion in the mpt64 gene and the other possessed a 3,659-bp deletion from Rv1977 to Rv1981c, a region including the entire mpt64 gene.Conclusions: Capilia TB-Neo is a simple, rapid and highly sensitive test for identifying MTC, and showed better specificity than Capilia TB. However, Capilia TB-Neo still showed false-negative results with mpt64 mutations. The limitation should be recognized for clinical use.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"BMC Infectious Diseases, 14(1), 54; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/1471-2334-14-54","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 Chikamatsu et al.; licensee BioMed Central Ltd."},{"subitem_rights":"This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14712334","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chikamatsu, Kinuyo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aono, Akio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugamoto, Tetsuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kazumi, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tamai, Kiyoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagisawa, Hideji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitarai, Satoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"BMCID14_54.pdf","filesize":[{"value":"263.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BMCID14_54.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5219/files/BMCID14_54.pdf"},"version_id":"211c788a-c44d-4623-bbbc-6819c8ee323a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Capilia TB-Neo","subitem_subject_scheme":"Other"},{"subitem_subject":"mpt64 gene","subitem_subject_scheme":"Other"},{"subitem_subject":"Mycobacterium tuberculosis complex identification","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-04-15"},"publish_date":"2014-04-15","publish_status":"0","recid":"5219","relation_version_is_last":true,"title":["Comparative evaluation of three immunochromatographic identification tests for culture confirmation of Mycobacterium tuberculosis complex"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:09:50.968718+00:00"}