{"created":"2023-05-15T16:33:07.066939+00:00","id":5226,"links":{},"metadata":{"_buckets":{"deposit":"79b11a0c-c209-4cdf-af9c-a71f541f91bb"},"_deposit":{"created_by":2,"id":"5226","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5226"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005226","sets":["35:36"]},"author_link":["21833","21826","21831","21834","21828","21832","21830","21829","21827"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"e2644","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"PLoS Neglected Tropical Diseases"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background\nWe previously identified a novel gene family dispersed in the genome of Schistosoma japonicum by retrotransposon-mediated gene duplication mechanism. Although many transcripts were identified, no homolog was readily identifiable from sequence information.\nMethodology/Principal Findings\nHere, we utilized structural homology modeling and biochemical methods to identify remote homologs, and characterized the gene products as SEA (sea-urchin sperm protein, enterokinase and agrin)-domain containing proteins. A common extracellular domain in this family was structurally similar to SEA-domain. SEA-domain is primarily a structural domain, known to assist or regulate binding to glycans. Recombinant proteins from three members of this gene family specifically interacted with glycosaminoglycans with high affinity, with potential implication in ligand acquisition and immune evasion. Similar approach was used to identify a heme-binding site on the SEA-domain. The heme-binding mode showed heme molecule inserted into a hydrophobic pocket, with heme iron putatively coordinated to two histidine axial ligands. Heme-binding properties were confirmed using biochemical assays and UV-visible absorption spectroscopy, which showed high affinity heme-binding (KD = 1.605×10?6 M) and cognate spectroscopic attributes of hexa-coordinated heme iron. The native proteins were oligomers, antigenic, and are localized on adult worm teguments and gastrodermis; major host-parasite interfaces and site for heme detoxification and acquisition.\nConclusions\nThe results suggest potential role, at least in the nucleation step of heme crystallization (hemozoin formation), and as receptors for heme uptake. Survival strategies exploited by parasites, including heme homeostasis mechanism in hemoparasites, are paramount for successful parasitism. Thus, assessing prospects for application in disease intervention is warranted.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS Neglected Tropical Diseases, 8(1), e2644; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pntd.0002644","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 Mbanefo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19352735","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mbanefo, Evaristus Chibunna"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kikuchi, Mihoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Huy, Nguyen Tien"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shuaibu, Mohammed Nasir"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cherif, Mahamoud Sama"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yu, Chuanxin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wakao, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suda, Yasuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirayama, Kenji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoSNeg8_2644.pdf","filesize":[{"value":"9.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoSNeg8_2644.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5226/files/PLoSNeg8_2644.pdf"},"version_id":"a932a8ba-ca42-47ea-943d-795810c894d5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of a Gene Family Encoding SEA (Sea-urchin Sperm Protein, Enterokinase and Agrin)-Domain Proteins with Lectin-Like and Heme-Binding Properties from Schistosoma japonicum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of a Gene Family Encoding SEA (Sea-urchin Sperm Protein, Enterokinase and Agrin)-Domain Proteins with Lectin-Like and Heme-Binding Properties from Schistosoma japonicum"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-04-17"},"publish_date":"2014-04-17","publish_status":"0","recid":"5226","relation_version_is_last":true,"title":["Characterization of a Gene Family Encoding SEA (Sea-urchin Sperm Protein, Enterokinase and Agrin)-Domain Proteins with Lectin-Like and Heme-Binding Properties from Schistosoma japonicum"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:09:40.957353+00:00"}