{"created":"2023-05-15T16:33:13.245502+00:00","id":5368,"links":{},"metadata":{"_buckets":{"deposit":"a3920301-3e66-43c6-a2a7-d87f1904f8de"},"_deposit":{"created_by":2,"id":"5368","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5368"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005368","sets":["73:74"]},"author_link":["22665","22667","22666","22663","22664","22668","22669"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"575","bibliographicPageStart":"569","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We previously developed an in vivo tissue suction-mediated transfection method (denoted as the tissue suction method) for naked nucleic acids, such as plasmid DNA (pDNA) and small interfering RNA (siRNA), in mice. However, it remains unclear whether the suction pressure conditions affect the results of this method. Therefore, in the present study, we assembled a computer system to control the suction pressure and investigate the effects of the suction pressure conditions on the efficiency of the liver suction transfection of naked pDNA that encodes luciferase in mice. Using the developed system, we examined the effects of the minimum magnitude of the suction pressure, suction pressure waveform, and suction times of the luciferase expression level in mice livers. We determined that the liver suction method at -5 kPa was not only effective but also caused the lowest hepatic toxicity in mice. Additionally, the results indicated that the suction pressure waveform affects the luciferase expression levels, and a single period of suction on the targeted portion of the liver is sufficient for transfection. Thus, the developed system is useful for performing the tissue suction method with high accuracy and safety.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological and Pharmaceutical Bulletin, 37(4), pp.569-575; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.b13-00776","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 The Pharmaceutical Society of Japan."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09186158","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13475215","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Pharmaceutical Society of Japan"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shimizu, Kazunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhang, Guangyuan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Shigeru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taniguchi, Yota"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Kouji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashida, Mitsuru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Konishi, Satoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"BPBul37_569.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BPBul37_569.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5368/files/BPBul37_569.pdf"},"version_id":"835d4cf4-2254-491c-a975-e2ed91c4fa41"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Computer system","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"Mechanical stimulus","subitem_subject_scheme":"Other"},{"subitem_subject":"Naked DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"Tissue suction","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-05"},"publish_date":"2014-06-05","publish_status":"0","recid":"5368","relation_version_is_last":true,"title":["Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:06:17.129184+00:00"}