{"created":"2023-05-15T16:33:13.411600+00:00","id":5372,"links":{},"metadata":{"_buckets":{"deposit":"8d2c22a3-c16f-4866-8225-c671ea175cae"},"_deposit":{"created_by":2,"id":"5372","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5372"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005372","sets":["10:11"]},"author_link":["22700","22699","22703","22702","22701","22704"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"424","bibliographicPageStart":"418","bibliographicVolumeNumber":"105","bibliographic_titles":[{"bibliographic_title":"Cancer Science"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"RUNX3 is a tumor suppressor for a variety of cancers. RUNX3 suppresses the canonical Wnt signaling pathway by binding to the TCF4/β-catenin complex, resulting in the inhibition of binding of the complex to the Wnt target gene promoter. Here, we confirmed that RUNX3 suppressed Wnt signaling activity in several gastric cancer cell lines; however, we found that RUNX3 increased the Wnt signaling activity in KatoIII and SNU668 gastric cancer cells. Notably, RUNX3 expression increased the ratio of the Wnt signaling-high population in the KatoIII cells. although the maximum Wnt activation level of individual cells was similar to that in the control. As found previously, RUNX3 also binds to TCF4 and β-catenin in KatoIII cells, suggesting that these molecules form a ternary complex. Moreover, the ChIP analyses revealed that TCF4, β-catenin and RUNX3 bind the promoter region of the Wnt target genes, Axin2 and c-Myc, and the occupancy of TCF4 and β-catenin in these promoter regions is increased by the RUNX3 expression. These results suggest that RUNX3 stabilizes the TCF4/β-catenin complex on the Wnt target gene promoter in KatoIII cells, leading to activation of Wnt signaling. Although RUNX3 increased the Wnt signaling activity, its expression resulted in suppression of tumorigenesis of KatoIII cells, indicating that RUNX3 plays a tumor-suppressing role in KatoIII cells through a Wnt-independent mechanism. These results indicate that RUNX3 can either suppress or activate the Wnt signaling pathway through its binding to the TCF4/β-catenin complex by cell context-dependent mechanisms.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Cancer Science, 105(4), pp.418-424; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本癌学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/cas.12356","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association."},{"subitem_rights":"This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13479032","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Japanese Cancer Association"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ju, Xiaoli"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishikawa, Tomo-o"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Naka, Kazuhito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Kosei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Yoshiaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oshima, Masanobu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"CanSci105_418.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CanSci105_418.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5372/files/CanSci105_418.pdf"},"version_id":"a9fdf58d-5984-4c8f-8a0c-ec19a3dd75ed"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Gastric cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"RUNX3","subitem_subject_scheme":"Other"},{"subitem_subject":"TCF4","subitem_subject_scheme":"Other"},{"subitem_subject":"Wnt signaling","subitem_subject_scheme":"Other"},{"subitem_subject":"β-catenin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Context-dependent activation of Wnt signaling by tumor suppressor RUNX3 in gastric cancer cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Context-dependent activation of Wnt signaling by tumor suppressor RUNX3 in gastric cancer cells"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-05"},"publish_date":"2014-06-05","publish_status":"0","recid":"5372","relation_version_is_last":true,"title":["Context-dependent activation of Wnt signaling by tumor suppressor RUNX3 in gastric cancer cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:06:11.479168+00:00"}