{"created":"2023-05-15T16:33:13.712632+00:00","id":5379,"links":{},"metadata":{"_buckets":{"deposit":"cf8b65b0-1ad7-4e35-a895-7ff4c65d272a"},"_deposit":{"created_by":2,"id":"5379","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5379"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005379","sets":["66:67"]},"author_link":["22773","22770","22771","22772","22769","22774","22776","22768","22775"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"390","bibliographicPageStart":"381","bibliographicVolumeNumber":"55","bibliographic_titles":[{"bibliographic_title":"Journal of Radiation Research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The microcolony assay developed by Withers and Elkind has been a gold standard to assess the surviving fraction of small intestinal stem cells after exposure to high (?8 Gy) doses of ionizing radiation (IR), but is not applicable in cases of exposure to lower doses. Here, we developed a novel in vitro assay that enables assessment of the surviving fraction of small intestinal stem cells after exposure to lower IR doses. The assay includes in vitro culture of small intestinal stem cells, which allows the stem cells to develop into epithelial organoids containing all four differentiated cell types of the small intestine. We used Lgr5-EGFP-IRES-CreERT2/ROSA26-tdTomato mice to identify Lgr5+ stem cells and their progeny. Enzymatically dissociated single crypt cells from the duodenum and jejunum of mice were irradiated with 7.25, 29, 101, 304, 1000, 2000 and 4000 mGy of X-rays immediately after plating, and the number of organoids was counted on Day 12. Organoid-forming efficiency of irradiated cells relative to that of unirradiated controls was defined as the surviving fraction of stem cells. We observed a significant decrease in the surviving fraction of stem cells at ?1000 mGy. Moreover, fluorescence-activated cell sorting analyses and passage of the organoids revealed that proliferation of stem cells surviving IR is significantly potentiated. Together, the present study demonstrates that the in vitro assay is useful for quantitatively assessing the surviving fraction of small intestinal stem cells after exposure to lower doses of IR as compared with previous examinations using the microcolony assay.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Journal of Radiation Research, 55(2), pp.381-390; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本放射線影響学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jrr/rrt123","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2013 The Author 2014. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for TherapeuticRadiology and Oncology."},{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04493060","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13499157","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Japan Radiation Research Society"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamauchi, Motohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Otsuka, Kensuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kondo, Hisayoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hamada, Nobuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomita, Masanori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Masayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakasono, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwasaki, Toshiyasu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Kazuo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"JRR55_381.pdf","filesize":[{"value":"853.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JRR55_381.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5379/files/JRR55_381.pdf"},"version_id":"f49eb604-e6a1-44ec-8bb3-6876010d2c4b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"In vitro culture","subitem_subject_scheme":"Other"},{"subitem_subject":"Ionizing radiation","subitem_subject_scheme":"Other"},{"subitem_subject":"Lgr5","subitem_subject_scheme":"Other"},{"subitem_subject":"Organoid","subitem_subject_scheme":"Other"},{"subitem_subject":"Small intestinal stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Survival assay","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A novel in vitro survival assay of small intestinal stem cells after exposure to ionizing radiation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel in vitro survival assay of small intestinal stem cells after exposure to ionizing radiation"}]},"item_type_id":"2","owner":"2","path":["67"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-06"},"publish_date":"2014-06-06","publish_status":"0","recid":"5379","relation_version_is_last":true,"title":["A novel in vitro survival assay of small intestinal stem cells after exposure to ionizing radiation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:06:02.345791+00:00"}