{"created":"2023-05-15T16:33:15.296577+00:00","id":5416,"links":{},"metadata":{"_buckets":{"deposit":"82780c7c-5880-42f8-a43a-a77be1bc97a6"},"_deposit":{"created_by":2,"id":"5416","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5416"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005416","sets":["25:26"]},"author_link":["22942","22946","22950","22939","22952","22944","22945","22948","22951","22947","22953","22949","22954","22943","22941","22940"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"e86455","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cyanovirin-N (CVN) potently inhibits human immunodeficiency virus type 1 (HIV-1) infection, but both cytotoxicity and immunogenicity have hindered the translation of this protein into a viable therapeutic. A molecular docking analysis suggested that up to 12 residues were involved in the interaction of the reverse parallel CVN dimer with the oligosaccharide targets, among which Leu-1 was the most prominent hot spot residue. This finding provided a possible explanation for the lack of anti-HIV-1 activity observed with N-terminal PEGylated CVN. Therefore, linker-CVN (LCVN) was designed as a CVN derivative with a flexible and hydrophilic linker (Gly4Ser)3 at the N-terminus. The N-terminal α-amine of LCVN was PEGylated to create 10 K PEG-aldehyde (ALD)-LCVN. LCVN and 10 K PEG-ALD-LCVN retained the specificity and affinity of CVN for high mannose N-glycans. Moreover, LCVN exhibited significant anti-HIV-1 activity with attenuated cytotoxicity in the HaCaT keratinocyte cell line and MT-4 T lymphocyte cell lines. 10 K PEG-ALD-LCVN also efficiently inactivated HIV-1 with remarkably decreased cytotoxicity and pronounced cell-to-cell fusion inhibitory activity in vitro. The linker-extended CVN and the mono-PEGylated derivative were determined to be promising candidates for the development of an anti-HIV-1 agent. This derivatization approach provided a model for the PEGylation of biologic candidates without introducing point mutations.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 9(1), e86455; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0086455","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chen, Jia"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Huang, Dane"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chen, Wei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Guo, Chaowan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wei, Bo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wu, Chongchao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Peng, Zhou"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fan, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hou, Zhibo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fang, Yongsheng"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Yifei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitazato, Kaio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yu, Guoying"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zou, Chunbin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Qian, Chuiwen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Xiong, Sheng"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoS9_86455.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS9_86455.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5416/files/PLoS9_86455.pdf"},"version_id":"fc5dbb43-ebe3-42c4-9c6b-644908e12008"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand"}]},"item_type_id":"2","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-20"},"publish_date":"2014-06-20","publish_status":"0","recid":"5416","relation_version_is_last":true,"title":["Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:05:14.006222+00:00"}