@article{oai:nagasaki-u.repo.nii.ac.jp:00005446,
 author = {Miura, Seiyou and Khan, Khaleque Newaz and Kitajima, Michio and Hiraki, Koichi and Moriyama, Shingo and Masuzaki, Hideaki and Samejima, Tetsurou and Fujishita, Akira and Ishimaru, Tadayuki},
 issue = {10},
 journal = {Human Reproduction},
 month = {Oct},
 note = {Background: The association between uterine myoma and infertility is still controversial. The anatomical defect of endometrium by uterine fibroids could be a factor for reducing pregnancy rates and increasing miscarriage rates. However, pregnancy and implantation rates were found to be significantly lower in women with intramural myomas (IMMs), when there was no deformity of uterine cavity. This could be due to other biological factors such as increased accumulation of inflammatory cells within fibroid tissue and corresponding endometrium that might impair fertility. Therefore, we tried to investigate the pattern of macrophage (Mψ) accumulation in different uterine fibroids and the production of chemokine and prostaglandin (PG) by these tissues. Methods: The selection criteria of uterine fibroids were based on the classification of European Society of Hysteroscopy. Biopsy specimens were collected from respective nodules and autologous endometrium of 20 women with submucosal myoma (SMM), 29 women with IMM and 18 women with subserosal myoma (SSM). CD68 immunoreactive Mφs were identified in these tissues by immunohistochemistry. A fraction of corresponding tissues were homogenized, and levels of monocyte chemotactic protein-1 (MCP-1) and PGF 2α were measured by enzyme-linked immunosorbent assay (ELISA). Results: Mφ infiltration in the myoma nodule and corresponding endometrium of women with SMM and IMM was significantly higher than that of women with SSM or control women (P < 0.01 and P < 0.05, respectively). This tissue accumulation of inflammatory cells was independent of the sizes of the myoma nodules and phases of menstrual cycle. The tissue concentration of MCP-1 corresponded to increased Mφ infiltration and was significantly higher in women with SMM and IMM than that in women with SSM (P < 0.05 for each). A positive correlation was observed between MCP-1 concentration and accumulated Mψ numbers in the endometrium of women with SMM and IMM but not in women with SSM. The tissue levels of PGF2α were also significantly higher in the nodule and corresponding endometrium of women with SMM and IMM than that in SSM or control women (P < 0.05 for each). Conclusions: Higher production of MCP-1 could be responsible for the increased accumulation of Mψ in women with SMM and IMM. The augmented inflammatory reaction in endometrium and increased PGF 2α levels might be detrimental to reproductive outcome in women with SMM or IMM., Human Reproduction, 21(10), pp.2545-2554; 2006},
 pages = {2545--2554},
 title = {Differential infiltration of macrophages and prostaglandin production by different uterine leiomyomas},
 volume = {21},
 year = {2006}
}