{"created":"2023-05-15T16:33:16.650892+00:00","id":5447,"links":{},"metadata":{"_buckets":{"deposit":"aa6a9f96-e347-479d-9f15-2aea15e9e0df"},"_deposit":{"created_by":2,"id":"5447","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5447"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005447","sets":["29:30"]},"author_link":["23180","23181","23177","23175","23179","23174","23178","23176"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"60","bibliographicPageStart":"49","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"Human Reproduction"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: The different macromolecules as secreted by macrophages (M ) in the pelvic environment are believed to enhance the growth of endometriosis. However, the possible mediator that stimulates M for the production of different growth factors is not well described. Therefore, we investigated the possible production of hepatocyte growth factor (HGF) by the basal and lipopolysaccharide (LPS)-stimulated M derived from women with or without endometriosis. Methods: Using primary culture and 4-well chamber slides, adherent M immunoreactive to CD68 were isolated from the peritoneal fluid (PF) of 20 infertile women with endometriosis and 12 women without endometriosis. The proliferation of basal and LPS-treated M was investigated by the dimethylthiazole tetrazolioum bromide (MTT) assay. The production of HGF in the culture media of basal and LPS-stimulated M was examined by enyme-linked immunosorbent assay. The expression of mRNA for HGF and its receptor, c-Met, in the M was investigated by RT-PCR. The effect of HGF on the growth of endometrial cells and M was analysed by bromodeoxyuridine (BrdU) incorporation. Results: A >100 % increase in the proliferation of peritoneal M derived from women with endometriosis, and particularly of those harbouring dominant red lesions, was observed after treatment with LPS (P < 0.05). A 4- and 3-fold increase in the production of HGF was observed by the LPS-treated M derived from women with stage I-II endometriosis and stage III-IV endometriosis, respectively, when compared with non-LPS-treated M (P < 0.001). At the transcriptional level, we found a 5-fold increase in HGF mRNA expression in LPS-treated M versus basal M in women with endometriosis (P < 0.001). The BrdU incorporation study indicates that 10-100 ng/ml of HGF enhanced the growth of endometrial epithelial cells, stroma and M (?50% increase) derived from women with endometriosis (all P < 0.05). Conclusion: LPS could be an inflammatory mediator of macrophage stimulation in the pelvic microenvironment. Besides mesenchymal cells, HGF is also produced by peritoneal M and is possibly involved in the growth of endometriosis.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Human Reproduction, 20(1), pp.49-60; 2005","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/humrep/deh525","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c European Society of Human Reproduction and Embryology 2004; all rights reserved."},{"subitem_rights":"This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 20(1), pp.49-60; 2005 is available online at: http://dx.doi.org/10.1093/humrep/deh525"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14602350","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Khan, Khaleque Newaz"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuzaki, Hideaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujishita, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitajima, Michio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomoko, Kohno"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sekine, Ichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuyama, Toshifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishimaru, Tadayuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"HumRep20_49.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HumRep20_49.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5447/files/HumRep20_49.pdf"},"version_id":"28ce4612-4654-4323-b1a8-0323125ad79e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cell growth","subitem_subject_scheme":"Other"},{"subitem_subject":"Endometriosis","subitem_subject_scheme":"Other"},{"subitem_subject":"Hepatocyte growth factor","subitem_subject_scheme":"Other"},{"subitem_subject":"Lipopolysaccharide","subitem_subject_scheme":"Other"},{"subitem_subject":"Macrophage","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Regulation of hepatocyte growth factor by basal and stimulated macrophages in women with endometriosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulation of hepatocyte growth factor by basal and stimulated macrophages in women with endometriosis"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-07-03"},"publish_date":"2014-07-03","publish_status":"0","recid":"5447","relation_version_is_last":true,"title":["Regulation of hepatocyte growth factor by basal and stimulated macrophages in women with endometriosis"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:04:35.105811+00:00"}