{"created":"2023-05-15T16:33:19.758080+00:00","id":5518,"links":{},"metadata":{"_buckets":{"deposit":"3cec009a-fd25-4c81-8899-3aeda3113d86"},"_deposit":{"created_by":2,"id":"5518","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5518"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005518","sets":["73:74"]},"author_link":["23502","23498","23495","23499","23501","23497","23500","23504","23503","23496"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-01-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"e00958-13","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"mBio"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Herpes simplex virus type 1 (HSV-1) establishes latency in neurons and can cause severe disseminated infection with neurological impairment and high mortality. This neurodegeneration is thought to be tightly associated with virus-induced cytoskeleton disruption. Currently, the regulation pattern of the actin cytoskeleton and the involved molecular mechanisms during HSV-1 entry into neurons remain unclear. Here, we demonstrate that the entry of HSV-1 into neuronal cells induces biphasic remodeling of the actin cytoskeleton and an initial inactivation followed by the subsequent activation of cofilin, a member of the actin depolymerizing factor family that is critical for actin reorganization. The disruption of F-actin dynamics or the modulation of cofilin activity by mutation, knockdown, or overexpression affects HSV-1 entry efficacy and virus-mediated cell ruffle formation. Binding of the HSV-1 envelope initiates the epidermal growth factor receptor (EGFR)-phosphatidylinositide 3-kinase (PI3K) signaling pathway, which leads to virus-induced early cofilin phosphorylation and F-actin polymerization. Moreover, the extracellular signal-regulated kinase (ERK) kinase and Rho-associated, coiled-coil-containing protein kinase 1 (ROCK) are recruited as downstream mediators of the HSV-1-induced cofilin inactivation pathway. Inhibitors specific for those kinases significantly reduce the virus infectivity without affecting virus binding to the target cells. Additionally, lipid rafts are clustered to promote EGFR-associated signaling cascade transduction. We propose that HSV-1 hijacks cofilin to initiate infection. These results could promote a better understanding of the pathogenesis of HSV-1-induced neurological diseases.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"mBio, 5(1), e00958-13; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1128/mBio.00958-13","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 Zheng et al."},{"subitem_rights":"This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"21507511","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zheng, Kai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Xiang, Yangfei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Xiao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Qiaoli"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhong, Meigong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Shaoxiang"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Xiaoyan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fan, Jianglin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitazato, Kaio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Yifei"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"mBio5_00958-13.pdf","filesize":[{"value":"5.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"mBio5_00958-13.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5518/files/mBio5_00958-13.pdf"},"version_id":"e3194790-344c-4dcb-a009-7489596201c4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Epidermal Growth Factor Receptor-PI3K Signaling Controls Cofilin Activity To Facilitate Herpes Simplex Virus 1 Entry into Neuronal Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Epidermal Growth Factor Receptor-PI3K Signaling Controls Cofilin Activity To Facilitate Herpes Simplex Virus 1 Entry into Neuronal Cells"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-07-28"},"publish_date":"2014-07-28","publish_status":"0","recid":"5518","relation_version_is_last":true,"title":["Epidermal Growth Factor Receptor-PI3K Signaling Controls Cofilin Activity To Facilitate Herpes Simplex Virus 1 Entry into Neuronal Cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:03:38.673021+00:00"}