{"created":"2023-05-15T16:33:19.973994+00:00","id":5523,"links":{},"metadata":{"_buckets":{"deposit":"a16cba71-d426-4e64-b77a-e4545f34fef2"},"_deposit":{"created_by":2,"id":"5523","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5523"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005523","sets":["29:30"]},"author_link":["23546","23545","23544","23543","23542","23548","23547"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"49","bibliographicPageStart":"41","bibliographicVolumeNumber":"92","bibliographic_titles":[{"bibliographic_title":"Microvascular Research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The effects of hepatocyte growth factor (HGF) on barrier functions were investigated by a blood-brain barrier (BBB) in vitro model comprising a primary culture of rat brain capillary endothelial cells (RBEC). In order to examine the response of the peripheral endothelial cells to HGF, human umbilical vascular endothelial cells (HUVEC) and human dermal microvascular endothelial cells (HMVEC) were also treated with HGF. HGF decreased the permeability of RBEC to sodium fluorescein and Evans blue albumin, and dose-dependently increased transendothelial electrical resistance (TEER) in RBEC. HGF altered the immunochemical staining pattern of F-actin bands and made ZO-1 staining more distinct on the linear cell borders in RBEC. In contrast, HGF increased sodium fluorescein and Evans blue albumin permeability in HMVEC and HUVEC, and decreased TEER in HMVEC. In HMVEC, HGF reduced cortical actin bands and increased stress fiber density, and increased the zipper-like appearance of ZO-1 staining. Western blot analysis showed that HGF significantly increased the amount of ZO-1 and VE-cadherin. HGF seems to act on the BBB to strengthen BBB integrity. These findings indicated that cytoskeletal rearrangement and cell-cell adhesion, such as through VE-cadherin and ZO-1, are candidate mechanisms for the influence of HGF on the BBB. The possibility that HGF has therapeutic significance in protecting the BBB from damage needs to be considered.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Microvascular Research, 92, pp.41-49; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Academic Press Inc."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.mvr.2013.12.004","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2013 Elsevier Inc."},{"subitem_rights":"NOTICE: this is the author’s version of a work that was accepted for publication in Microvascular Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microvascular Research, 92, (2014)"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00262862","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamada, Narumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Shinsuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Horai, Shoji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Kunihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Deli, Maria A."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yatsuhashi, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niwa, Masami"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"MicRes92_4.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MicRes92_4.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5523/files/MicRes92_4.pdf"},"version_id":"70403ae6-c92f-43aa-8437-3ce456a27381"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hepatocyte growth factor","subitem_subject_scheme":"Other"},{"subitem_subject":"Blood-brain barrier","subitem_subject_scheme":"Other"},{"subitem_subject":"Tight junctions","subitem_subject_scheme":"Other"},{"subitem_subject":"Adherens junction","subitem_subject_scheme":"Other"},{"subitem_subject":"F-actin, ZO-1","subitem_subject_scheme":"Other"},{"subitem_subject":"VE-cadherin","subitem_subject_scheme":"Other"},{"subitem_subject":"Peripheral endothelial cells","subitem_subject_scheme":"Other"},{"subitem_subject":"TEER","subitem_subject_scheme":"Other"},{"subitem_subject":"permeability","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-07-31"},"publish_date":"2014-07-31","publish_status":"0","recid":"5523","relation_version_is_last":true,"title":["Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:03:30.573255+00:00"}