{"created":"2023-05-15T16:33:26.867794+00:00","id":5684,"links":{},"metadata":{"_buckets":{"deposit":"5ba5edcd-26c8-4e20-b4a3-801cbaeacf49"},"_deposit":{"created_by":2,"id":"5684","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5684"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005684","sets":["29:30"]},"author_link":["24282","24283","24284","24285","24286","24287","24281","24280"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-08-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"6006","bibliographicVolumeNumber":"4","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"As a prompt response against invasion of various viruses, interferon regulatory factor-3 (IRF-3) is initially phosphorylated to become activated and upregulates mainly Type I Interferons (IFN-I) in most cell types. We previously reported that IRF-3-dependent host innate immune responses partially interfere in infection of prions. Here, we found that stable infection of prion suppressed IRF-3 gene-expression. The decreased promoter activity of IRF-3 was significantly restored along with treatment of anti-prion drugs in the prion-infected cells, suggesting that infection of prion directly influence the regulation of IRF-3 transcription. We further investigated promoter activity of 5'- flanking region of murine IRF-3 using a luciferase reporter system and found that the nucleotides -119 to -1 were indispensable for the promoter activity. Within this region, mutations in the Oct-1 binding site significantly reduced the promoter activity and chromatin immunoprecipitation (ChIP) assay revealed that Oct-1 indeed binds to the region. In addition, overexpression of Oct-1 increased the promoter activity of IRF-3. Intriguingly, Oct-1 protein was significantly reduced in prion-infected cells and mice brains compared with uninfected groups. Taken together, we concluded that prion infection could interfere in the function of Oct-1, resulting in the down-regulation of IRF-3.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Scientific Reports, 4, 6006; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/srep06006","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Homma, Takujiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishibashi, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagaki, Takehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fuse, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sano, Kazunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Satoh, Katsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Atarashi, Ryuichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishida, Noriyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"SciRep4_6006.pdf","filesize":[{"value":"682.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SciRep4_6006.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5684/files/SciRep4_6006.pdf"},"version_id":"eb343b46-1ffe-40b5-8929-38437bbf5ee7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Persistent prion infection disturbs the function of Oct-1, resulting in the down-regulation of murine interferon regulatory factor-3","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Persistent prion infection disturbs the function of Oct-1, resulting in the down-regulation of murine interferon regulatory factor-3"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-10"},"publish_date":"2014-10-10","publish_status":"0","recid":"5684","relation_version_is_last":true,"title":["Persistent prion infection disturbs the function of Oct-1, resulting in the down-regulation of murine interferon regulatory factor-3"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:00:49.414091+00:00"}