{"created":"2023-05-15T16:33:26.910044+00:00","id":5685,"links":{},"metadata":{"_buckets":{"deposit":"74302b20-3497-42a3-9187-b01273bd161d"},"_deposit":{"created_by":2,"id":"5685","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5685"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005685","sets":["50:51"]},"author_link":["24294","24288","24293","24292","24290","24291","24289"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-07-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"4164","bibliographicPageStart":"4148","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Marine Drugs"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Marine-derived sulfated polysaccharides have been shown to possess certain anti-virus, anti-tumor, anti-inflammatory and anti-coagulant activities. However, the in vivo immunomodulatory effects of marine-derived pure compounds have been less well characterized. In this study, we investigated the effect of ascophyllan, a sulfated polysaccharide purified from Ascophyllum nodosum, on the maturation of mouse dendritic cells (DCs) in vitro and in vivo. Ascophyllan induced up-regulation of co-stimulatory molecules and production of pro-inflammatory cytokines in bone marrow-derived DCs (BMDCs). Moreover, in vivo administration of ascophyllan promotes up-regulation of CD40, CD80, CD86, MHC class I and MHC class II and production of IL-6, IL-12 and TNF-α in spleen cDCs. Interestingly, ascophyllan induced a higher degree of co-stimulatory molecule up-regulation and pro-inflammatory cytokine production than fucoidan, a marine-derived polysaccharide with well-defined effect for promoting DC maturation. Ascophyllan also promoted the generation of IFN-γ-producing Th1 and Tc1 cells in the presence of DCs in an IL-12-dependent manner. Finally, myeloid differentiation primary response 88 (MyD88) signaling pathway was essential for DC maturation induced by ascophyllan. Taken together, these results demonstrate that ascophyllan induces DC maturation, and consequently enhances Th1 and Tc1 responses in vivo. This knowledge could facilitate the development of novel therapeutic strategies to combat infectious diseases and cancer.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Marine Drugs, 12(7), pp.4148-4164; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Multidisciplinary Digital Publishing Institute (MDPI)"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/md12074148","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"16603397","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zhang, Wei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Du, Jiang-Yuan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jiang, Zedong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okimura, Takasi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oda, Tatsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yu, Qing"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jin, Jun-O"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"MarDru12_4148.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MarDru12_4148.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5685/files/MarDru12_4148.pdf"},"version_id":"faaae00f-0805-4c81-ba51-7ea284e44dc8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ascophyllan","subitem_subject_scheme":"Other"},{"subitem_subject":"DC maturation","subitem_subject_scheme":"Other"},{"subitem_subject":"Dendritic cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Tc1 cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Th1 cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ascophyllan Purified from Ascophyllum nodosum Induces Th1 and Tc1 Immune Responses by Promoting Dendritic Cell Maturation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ascophyllan Purified from Ascophyllum nodosum Induces Th1 and Tc1 Immune Responses by Promoting Dendritic Cell Maturation"}]},"item_type_id":"2","owner":"2","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-10"},"publish_date":"2014-10-10","publish_status":"0","recid":"5685","relation_version_is_last":true,"title":["Ascophyllan Purified from Ascophyllum nodosum Induces Th1 and Tc1 Immune Responses by Promoting Dendritic Cell Maturation"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T03:00:48.074648+00:00"}