{"created":"2023-05-15T16:33:34.938885+00:00","id":5869,"links":{},"metadata":{"_buckets":{"deposit":"215a7257-72ae-4967-8330-e833059afc7c"},"_deposit":{"created_by":2,"id":"5869","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5869"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005869","sets":["35:36"]},"author_link":["25218","25219","25222","25223","25220","25221","25225","25224","25217"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-10-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageStart":"e110777","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Among pneumococcal serotypes, some serotypes are more prevalent in the nasopharynx than others; determining factors for higher prevalence remain to be fully explored. As non-vaccine serotypes have emerged after the introduction of 7-valent conjugate vaccines, study of serotype specific epidemiology is in need. When two or more serotypes co-colonize, they evolve rapidly to defend host's immune responses; however, a clear association of cocolonization with a clinical outcome is lacking. Methods: Children less than 5 years old who were admitted to hospital due to acute respiratory infections (ARI) (n = 595) and healthy children (n = 350) were recruited. Carriage of pneumococcus was determined by culture and lytA PCR in the nasopharyngeal samples. Serotype/serogroup detection and its quantification were done by the nanofluidic real time PCR system. Spearman's correlation and logistic regression were used to examine a correlation of serotype/serogroup specific bacterial load with its prevalence and an association of co-colonization with ARI respectively. Results: Serotype/serogroup specific bacterial load was correlated with its prevalence, both in ARI cases (Spearman's rho = 0.44, n = 186; P<0.0001) and healthy children (Spearman's rho = 0.41, n = 115; P<0.0001). The prevalence of multiple serotypes was more common in ARI cases than in healthy children (18.5% vs 7.1%; aOR 2.92, 95% CI: 1.27-6.71; P = 0.01). The dominant serotype in the co-colonization had a 2 log10 higher bacterial load than the subdominant serotype, both in ARI cases (P<0.001) and healthy children (P<0.05). Conclusions: High bacterial load in the nasopharynx may help transmit pneumococci among hosts, and increase the chance of successful acquisition and colonization. Co-colonization of multiple serotypes of pneumococci is linked with ARI, which infers the interactions of multiple serotypes may increase their pathogenicity; however, they may compete for growth in number.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 9(10), e110777; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0110777","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 Dhoubhadel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Dhoubhadel, Bhim Gopal"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasunami, Michio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nguyen, Hien Anh Thi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Motoi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Vu, Thu Huong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Thi, Thuy Nguyen  Ai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dang, Duc Anh"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Lay-Myint"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ariyoshi, Koya"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoS9_110777.pdf","filesize":[{"value":"789.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS9_110777.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5869/files/PLoS9_110777.pdf"},"version_id":"cdb2e7f5-b49b-4241-9a94-e9df9f8f0be8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bacterial Load of Pneumococcal Serotypes Correlates with Their Prevalence and Multiple Serotypes Is Associated with Acute Respiratory Infections among Children Less Than 5 Years of Age","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bacterial Load of Pneumococcal Serotypes Correlates with Their Prevalence and Multiple Serotypes Is Associated with Acute Respiratory Infections among Children Less Than 5 Years of Age"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-12-17"},"publish_date":"2014-12-17","publish_status":"0","recid":"5869","relation_version_is_last":true,"title":["Bacterial Load of Pneumococcal Serotypes Correlates with Their Prevalence and Multiple Serotypes Is Associated with Acute Respiratory Infections among Children Less Than 5 Years of Age"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:57:25.973686+00:00"}