{"created":"2023-05-15T16:33:37.142407+00:00","id":5920,"links":{},"metadata":{"_buckets":{"deposit":"3ab4dfc9-0090-45d2-a237-1d61eaec6026"},"_deposit":{"created_by":2,"id":"5920","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5920"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005920","sets":["35:36"]},"author_link":["25614","25609","25611","25612","25616","25615","25617","25610","25613"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"3962","bibliographicPageStart":"3957","bibliographicVolumeNumber":"113","bibliographic_titles":[{"bibliographic_title":"Parasitology Research"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Malaria parasites are under oxidative attack throughout their life cycle in human body and mosquito vector. Therefore, Plasmodium antioxidant defenses are crucial for its survival and being considered as interesting target for antimalarial drug design. Plasmodium knowlesi has emerged recently from its simian host to human in Southeast Asia and has been recognized as the fifth Plasmodium species that can cause human malaria. In this study, we cloned and characterized thioredoxin peroxidase 1 from P. knowlesi (PkTPx-1). PkTPx-1 gene was cloned, and recombinant protein was produced by heterologous overexpression in Escherichia coli. The recombinant protein was used for evaluation of enzymatic activity and polyclonal antibody production. Using the recombinant PkTPx-1 protein, its antioxidant activity was confirmed in a mixed-function oxidation assay where PkTPx-1 prevented nicking of DNA by hydroxyl radicals. PkTPx-1 was able to bind to double-strand DNA and RNA and had RNA chaperone activity in a nucleic acid melting assay indicating new function of PkTPx-1 other than antioxidant activity. Using specific polyclonal antibodies, it was indicated that PkTPx-1 is expressed in the cytoplasm of the parasite. Altogether, these results suggest that PkTPx-1 not only protects the parasite from the adverse effects of reactive oxygen species but also has RNA chaperone activity.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Parasitology Research, 113(11), pp.3957-3962; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Verlag"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00436-014-4060-0","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c Springer-Verlag Berlin Heidelberg 2014"},{"subitem_rights":"The final publication is available at link.springer.com"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09320113","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14321955","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hakimi, Hassan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suganuma, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Usui, Miho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuda-Suganuma, Hirono"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Angeles, Jose Ma. M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Asada, Masahito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawai, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Noboru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawazu, Shin-ichiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"ParRes113_3957.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ParRes113_3957.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5920/files/ParRes113_3957.pdf"},"version_id":"e9e1b9aa-99cc-429e-922a-48b0e0881065"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Nucleic acid binding","subitem_subject_scheme":"Other"},{"subitem_subject":"Plasmodium knowlesi","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA chaperone","subitem_subject_scheme":"Other"},{"subitem_subject":"Thioredoxin peroxidase 1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity"}]},"item_type_id":"2","owner":"2","path":["36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-12-01"},"publish_date":"2015-12-01","publish_status":"0","recid":"5920","relation_version_is_last":true,"title":["Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:56:16.288177+00:00"}