{"created":"2023-05-15T16:33:37.854367+00:00","id":5937,"links":{},"metadata":{"_buckets":{"deposit":"3381fbea-b361-44b6-9102-4c7b4d33f18c"},"_deposit":{"created_by":2,"id":"5937","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"5937"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00005937","sets":["25:26"]},"author_link":["25751","25759","25754","25757","25756","25762","25761","25758","25763","25753","25755","25752","25760"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-12-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageStart":"e115121","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background and Purpose: To understand the mechanisms involved in the strong killing effect of carbon-ion beam irradiation on cancer cells with TP53 tumor suppressor gene deficiencies.Copyright:\nMaterials and Methods: DNA damage responses after carbon-ion beam or X-ray irradiation in isogenic HCT116 colorectal cancer cell lines with and without TP53 (p53+/ + and p53-/-, respectively) were analyzed as follows: cell survival by clonogenic assay, cell death modes by morphologic observation of DAPI-stained nuclei, DNA doublestrand breaks (DSBs) by immunostaining of phosphorylated H2AX (γH2AX), and cell cycle by flow cytometry and immunostaining of Ser10-phosphorylated histone H3.\nResults: The p53-/- cells were more resistant than the p53+/+ cells to X-ray irradiation, while the sensitivities of the p53+/+ and p53-/- cells to carbon-ion beam irradiation were comparable. X-ray and carbon-ion beam irradiations predominantly induced apoptosis of the p53+/+ cells but not the p53-/- cells. In the p53-/- cells, carbon-ion beam irradiation, but not X-ray irradiation, markedly induced mitotic catastrophe that was associated with premature mitotic entry with harboring longretained DSBs at 24 h post-irradiation.\nConclusions: Efficient induction of mitotic catastrophe in apoptosis-resistant p53- deficient cells implies a strong cancer cell-killing effect of carbon-ion beam irradiation that is independent of the p53 status, suggesting its biological advantage over X-ray treatment.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 9(12), e115121; 2014","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0115121","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2014 Amornwichet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Amornwichet, Napapat"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oike, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogiwara, Hideaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuchiya, Naoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamauchi, Motohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saitoh, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sekine, Ryota"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Isono, Mayu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Yukari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohno, Tatsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kohno, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakano, Takashi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoS9_115121.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS9_115121.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/5937/files/PLoS9_115121.pdf"},"version_id":"432165c0-1fd7-41cf-a0bb-45d553db1dbc"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Carbon-Ion Beam Irradiation Kills X-Ray-Resistant p53-Null Cancer Cells by Inducing Mitotic Catastrophe","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Carbon-Ion Beam Irradiation Kills X-Ray-Resistant p53-Null Cancer Cells by Inducing Mitotic Catastrophe"}]},"item_type_id":"2","owner":"2","path":["26"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-01-23"},"publish_date":"2015-01-23","publish_status":"0","recid":"5937","relation_version_is_last":true,"title":["Carbon-Ion Beam Irradiation Kills X-Ray-Resistant p53-Null Cancer Cells by Inducing Mitotic Catastrophe"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:55:55.939638+00:00"}