{"created":"2023-05-15T16:29:44.183953+00:00","id":600,"links":{},"metadata":{"_buckets":{"deposit":"bd023f0f-772a-455a-9dc4-b40a6e67798c"},"_deposit":{"created_by":2,"id":"600","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"600"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000600","sets":["50:51"]},"author_link":["3096","3097","3095","3093","3094","3092","3091","3090"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-01-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"771","bibliographicPageStart":"753","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"Fish Physiology and Biochemistry"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The localization of gonadotropin-releasing hormone (GnRH) in the brain and pituitary of the self-fertilizing mangrove killifish Kryptolebias marmoratus was examined by immunohistochemistry and in situ hybridization to understand its neuroendocrine system. The genome assembly of K. marmoratus did not have any sequence encoding GnRH1, but sequences encoding GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH) were found. Therefore, GnRH1 was identified by in silico cloning. The deduced amino acid sequence of the K. marmoratus GnRH1 (mature peptide) was identical to that of the medaka GnRH. GnRH1 neurons were detected in the ventral part of the preoptic nucleus by immunohistochemistry and in situ hybridization, and GnRH1-immunoreactive (ir) fibers were observed throughout the brain. GnRH1-ir fibers were in close contact with luteinizing hormone (LH)-ir cells in the pituitary using double immunohistochemistry. GnRH2 neurons were detected in the midbrain tegmentum by immunohistochemistry and in situ hybridization. Although GnRH2-ir fibers were observed throughout the brain, they were not detected in the pituitary. GnRH3 neurons were detected in the lateral part of the ventral telencephalic area by both methods. GnRH3-ir fibers were observed throughout the brain, and a few GnRH3-ir fibers were in close contact with LH-ir cells in the pituitary. These results indicate that GnRH1 and possibly GnRH3 are responsible for gonadal maturation through LH secretion and that all three forms of GnRH function as neurotransmitters or neuromodulators in the brain of K. marmoratus.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Fish Physiology and Biochemistry, 45(2), pp.753-771; 2019","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10695-018-0601-2","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c Springer Nature B.V. 2019 This is a post-peer-review, pre-copyedit version of an article published in Fish Physiology and Biochemistry. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10695-018-0601-2."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09201742","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"15735168","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Amano, Masafumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Amiya, Noriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okubo, Kataaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Junpei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuriu, Ayae"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuta, Ayano"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Naoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakakura, Yoshitaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-18"}],"displaytype":"detail","filename":"FPB45_753.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"FPB45_753.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/600/files/FPB45_753.pdf"},"version_id":"41ef0911-d9e7-477a-9e7b-10a41edcc0d4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GnRH","subitem_subject_scheme":"Other"},{"subitem_subject":"GTH","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_scheme":"Other"},{"subitem_subject":"In situ hybridization","subitem_subject_scheme":"Other"},{"subitem_subject":"Self-fertilizing fish","subitem_subject_scheme":"Other"},{"subitem_subject":"Brain","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus"}]},"item_type_id":"2","owner":"2","path":["51"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-04-02"},"publish_date":"2020-04-02","publish_status":"0","recid":"600","relation_version_is_last":true,"title":["Localization of three forms of gonadotropin-releasing hormone in the brain and pituitary of the self-fertilizing fish, Kryptolebias marmoratus"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T04:14:45.467986+00:00"}