@article{oai:nagasaki-u.repo.nii.ac.jp:00006020,
 author = {Ohyama, Kaname and Baba, Miyako and Tamai, Mami and Aibara, Nozomi and Ichinose, Kunihiro and Kishikawa, Naoya and Kawakami, Atsushi and Kuroda, Naotaka},
 issue = {3},
 journal = {Clinical Biochemistry},
 month = {Feb},
 note = {Objective: Immune complexes (ICs) trigger humoral immune responses. Therefore, the identification of constituent antigens within ICs would have very different clinical significance than identification of free antigens. Design and methods: Here, we applied immune complexome analysis of serum to the study of seven major autoimmune diseases-anti-neutrophil cytoplasmic antibody-associated vasculitis, Takayasu's arteritis, mixed connective tissue disease, dermatomyositis, Sjogren's syndrome, systemic scleroderma, and systemic lupus erythematosus-and healthy donors to comprehensively identify antigens incorporated into circulating ICs and to find disease-specific antigens. Results: We identified 468 distinct IC-associated antigens using this method. Importantly, 62 of those antigens were disease-specific antigens, and there were at least three disease-specific antigens for each of the seven autoimmune diseases. Of the disease-specific antigens identified, coiled-coil domain-containing protein 158 and spectrin were identified as potential autoantigens important to SSc and SS pathogenesis, respectively; notable titin and spectrin autoantibodies are reportedly found in SSc and SS patients, respectively. Conclusion: Immune complexome analysis may be generally applicable to the study of the relationship between ICs and autoimmune diseases in animals and humans., Clinical Biochemistry, 48(3), pp.181-185; 2015},
 pages = {181--185},
 title = {Proteomic profiling of antigens in circulating immune complexes associated with each of seven autoimmune diseases},
 volume = {48},
 year = {2015}
}