{"created":"2023-05-15T16:29:44.484331+00:00","id":607,"links":{},"metadata":{"_buckets":{"deposit":"080d7071-8001-495d-9f92-d6f98169058d"},"_deposit":{"created_by":6,"id":"607","owners":[6],"pid":{"revision_id":0,"type":"depid","value":"607"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00000607","sets":["10:22:24"]},"author_link":["116700","116699"],"item_5_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Prevotella intermedia ジペプチダーゼAの基質特異性の決定と新規オートプロセシング機構"}]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"642","bibliographicPageStart":"629","bibliographicVolumeNumber":"401","bibliographic_titles":[{"bibliographic_title":"Biological Chemistry"}]}]},"item_5_date_granted_64":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-03-19"}]},"item_5_degree_grantor_62":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Nagasaki University (長崎大学)"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17301","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_61":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)"}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Prevotella intermedia, a gram-negative anaerobic rod, is frequently observed in subgingival polymicrobial biofilm from adults with chronic periodontitis. Peptidases in periodontopathic bacteria are considered to function as etiological reagents. Pre. intermedia OMA14 cells abundantly express an unidentified cysteine peptidase specific for Arg-4-methycoumaryl-7- amide (MCA). BAU17746 (locus tag, PIOMA14_I_1238) and BAU18827 (locus tag, PIOMA14_II_0322) emerged as candidates of this peptidase from the substrate specificity and sequence similarity with C69-family Streptococcus gordonii Arg-aminopeptidase. The recombinant form of the former solely exhibited hydrolyzing activity toward Arg-MCA, and BAU17746 possesses a 26.6% amino acid identity with the C69-family Lactobacillus helveticus dipeptidase A. It was found that BAU17746 as well as L. helveticus dipeptidase A was a P1-position Arg-specific dipeptidase A, although the L. helveticus entity, a representative of the C69 family, had been reported to be specific for Leu and Phe. The fulllength form of BAU17746 was intramolecularly processed to a mature form carrying the N-terminus of Cys15. In conclusion, the marked Arg-MCA-hydrolyzing activity in Pre. intermedia was mediated by BAU17746 belonging to the C69-family dipeptidase A, in which the mature form carries an essential cysteine at the N-terminus.","subitem_description_type":"Abstract"}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"長崎大学学位論文 学位記番号:博(医歯薬)甲第1215号 学位授与年月日:令和2年3月19日","subitem_description_type":"Other"},{"subitem_description":"Author: Mohammad Tanvir Sarwar, Yuko Ohara-Nemoto, Takeshi Kobayakawa, Mariko Naito and Takayuki K. Nemoto","subitem_description_type":"Other"},{"subitem_description":"Citation: Biological Chemistry, 401(5),  pp. 629-642 ; 2020","subitem_description_type":"Other"}]},"item_5_description_69":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Nagasaki University (長崎大学), 博士(歯学) (2020-03-19)","subitem_description_type":"Other"}]},"item_5_dissertation_number_65":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医歯薬第1215号"}]},"item_5_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"116700","nameIdentifierScheme":"WEKO"}],"names":[{"name":"サルワル, モハマド　タンビール"}]}]},"item_5_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Walter de Gruyter"}]},"item_5_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1515/hsz-2019-0387","subitem_relation_type_select":"DOI"}}]},"item_5_relation_42":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10069/39913","subitem_relation_type_select":"HDL"}}]},"item_5_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"c 2020 by Walter de Gruyter Berlin/Boston. The final publication is available at www.degruyter.com"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14316730","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14374315","subitem_source_identifier_type":"ISSN"}]},"item_5_text_67":{"attribute_name":"学位の種類","attribute_value_mlt":[{"subitem_text_value":"課程博士"}]},"item_5_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SARWAR, MOHAMMAD TANVIR"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-02"}],"displaytype":"detail","filename":"ISYK1215_Sarwar.pdf","filesize":[{"value":"3.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISYK1215_Sarwar.pdf","objectType":"fulltext","url":"https://nagasaki-u.repo.nii.ac.jp/record/607/files/ISYK1215_Sarwar.pdf"},"version_id":"bdfb2105-f76c-4170-bde9-b57e90180579"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"dipeptidase A","subitem_subject_scheme":"Other"},{"subitem_subject":"cysteine peptidase","subitem_subject_scheme":"Other"},{"subitem_subject":"autoprocessing","subitem_subject_scheme":"Other"},{"subitem_subject":"Prevotella intermedia","subitem_subject_scheme":"Other"},{"subitem_subject":"substrate specificity","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontal disease","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia","subitem_title_language":"en"}]},"item_type_id":"5","owner":"6","path":["24"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-04-07"},"publish_date":"2020-04-07","publish_status":"0","recid":"607","relation_version_is_last":true,"title":["Characterization of substrate specificity and novel autoprocessing mechanism of dipeptidase A from Prevotella intermedia"],"weko_creator_id":"6","weko_shared_id":-1},"updated":"2023-08-23T04:57:51.773106+00:00"}