@article{oai:nagasaki-u.repo.nii.ac.jp:00006091,
 author = {Yamashita, Yoshiro and Hoshino, Yoshihiko and Oka, Mayuko and Matsumoto, Sokichi and Ariga, Haruyuki and Nagai, Hideaki and Makino, Masahiko and Ariyoshi, Koya and Tsunetsugu-Yokota, Yasuko},
 issue = {3},
 journal = {Japanese Journal of Infectious Diseases},
 month = {May},
 note = {Although IFN-γ release assays (IγRAs) provide increased specificity over tuberculin skin tests, the early and sensitive detection of reactivation of latently infected Mycobacterium tuberculosis is required to control tuberculosis (TB). Recently, a multicolor flow cytometry has been developed to study CD4+ T cell cytokine responses (IFN-γ/IL-2/TNF-a) to purified protein derivatives (PPD) and M. tuberculosis-specific antigens (ESAT-6/CFP-10) and provided useful information regarding anti-TB immunity. However, the diagnostic relevancy remains uncertain. Here, we analyzed three additional CD4+ T cell cytokine responses (IL-10/IL-13/IL-17) to latent mycobacterial antigens (a-crystallin, methylated heparin-binding hemagglutinin [HBHA], and mycobacterial DNA-binding protein 1 [MDP-1]) as well as PPD and ESAT-6/CFP-10 in 12 IGRA+ TB cases and 8 healthy controls. No significant difference in IFN-γ response was observed between TB cases and controls, which was likely due to the high variation among the individuals. However, we found a significant increase over healthy controls in (i) the IL-2 response to HBHA in recovery stage TB cases, (ii) the number of M. tuberculosisspecific polyfunctional CD4+ T cells in on-treatment and recovery stage cases, and (iii) the IL-17 response to HBHA and MDP-1 in on-treatment and recovery stage cases. These results suggest that a combination of these T cell cytokine parameters could aid in accurate diagnosis of latent TB infection., Japanese Journal of Infectious Diseases, 66(3), pp.207-215; 2013},
 pages = {207--215},
 title = {Multicolor Flow Cytometric Analyses of CD4+ T Cell Responses to Mycobacterium tuberculosis-Related Latent Antigens},
 volume = {66},
 year = {2013}
}