{"created":"2023-05-15T16:33:49.438981+00:00","id":6210,"links":{},"metadata":{"_buckets":{"deposit":"666ca2cb-5465-49fe-a4c5-2b761937ae96"},"_deposit":{"created_by":2,"id":"6210","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"6210"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00006210","sets":["8:9"]},"author_link":["26874","26879","26873","26876","26870","26871","26875","26877","26878","26872"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"1001","bibliographicPageStart":"995","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Biological and Pharmaceutical Bulletin"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We developed binary and ternary complexes based on polymers and liposomes for safe and effective delivery of small interfering RNA (siRNA). Anti-luciferase siRNA was used as a model of nucleic acid medicine. The binary complexes of siRNA were prepared with cationic polymers and cationic liposomes such as polyethylenimine (PEI), polyamidoamine (PAMAM) dendrimer, poly-L-arginine (PLA), trimethyl[2,3-(dioleoxy)-propyl]ammonium chloride (DOTMA), and cholesteryl 3β-N-(dimetylaminnoethyl)carbamate hydrochloride (DC-Chol). The ternary complexes were constructed by the addition of γ-polyglutamic acid (γ-PGA) to the binary complexes. The complexes were approximately 54-153 nm in particle size. The binary complexes showed a cationic surface charge although an anionic surface charge was observed in the ternary complexes. The polymer-based complexes did not show a silencing effect in the mouse colon carcinoma cell line expressing luciferase regularly (Colon26/Luc cells). The binary complexes based on liposomes and their ternary complexes coated by γ-PGA showed a significant silencing effect. The binary complexes showed significant cytotoxicity although the ternary complexes coated by γ-PGA did not show significant cytotoxicity. The ternary complexes coated by γ-PGA suppressed luciferase activity in the tumor after their direct injection into the tumors of mice bearing Colon26/Luc cells. Thus, we have newly identified safe and efficient ternary complexes of siRNA for clinical use.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Biological and Pharmaceutical Bulletin, 36(6), pp.995-1001; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本薬学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1248/bpb.b13-00054","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 The Pharmaceutical Society of Japan"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09186158","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13475215","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Pharmaceutical Society of Japan"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kodama, Yukinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Harauchi, Satoe"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawanabe, Saki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ichikawa, Nobuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Hiroo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muro, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higuchi, Norihide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tadahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitahara, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hitoshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"BPBul36_995.pdf","filesize":[{"value":"838.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BPBul36_995.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/6210/files/BPBul36_995.pdf"},"version_id":"43054ae8-ebf3-4878-ab94-9c07779b0b24"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"γ-polyglutamic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"Cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"Cationic liposome","subitem_subject_scheme":"Other"},{"subitem_subject":"Cationic polymer","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"Small interfering RNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Safe and Effective Delivery of Small Interfering RNA with Polymer- and Liposomes-Based Complexes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Safe and Effective Delivery of Small Interfering RNA with Polymer- and Liposomes-Based Complexes"}]},"item_type_id":"2","owner":"2","path":["9"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-05"},"publish_date":"2013-08-05","publish_status":"0","recid":"6210","relation_version_is_last":true,"title":["Safe and Effective Delivery of Small Interfering RNA with Polymer- and Liposomes-Based Complexes"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:49:50.728791+00:00"}