{"created":"2023-05-15T16:34:01.529763+00:00","id":6478,"links":{},"metadata":{"_buckets":{"deposit":"c0104b06-b9f0-4c8d-b638-e81e9745eca7"},"_deposit":{"created_by":2,"id":"6478","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"6478"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00006478","sets":["73:74"]},"author_link":["27533","27531","27532","27534","27535"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"306","bibliographicPageStart":"301","bibliographicVolumeNumber":"31","bibliographic_titles":[{"bibliographic_title":"Forensic Toxicology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Paraquat (N,N′-dimethyl-4,4′-bipyridinium dichloride) is one of the most widely used herbicides owing to its high efficacy and low environmental persistence. However, because paraquat has significant acute toxicity, fatalities are often caused by accidental or voluntary ingestion of paraquat. In consideration of the strong toxicity and fast-Acting property of paraquat, on-site analysis at accident scenes should be effective in facilitating immediate medical treatment. In this study, a simple and rapid chemiluminescence assay using a portable luminometer was developed for on-site analysis of paraquat. The proposed assay is based on luminol chemiluminescence detection of superoxide anion radical resulting from the redox reaction between paraquat and dithiothreitol. Intense chemiluminescence was observed after mixing of paraquat and dithiothreitol in the presence of luminol. Because the chemiluminescence intensity was proportional to the concentration of paraquat, a quantitative measurement of paraquat was possible. The calibration curve for standard paraquat solution was linear from 0.025 to 2.5 μM with the correlation coefficient of 0.992; the detection limit (blank + 3SD) was 22 nM. The proposed assay was applied to determine paraquat in beverage samples after a cation exchange clean-up procedure. Given that the portable luminometer used in this study is small and lightweight, the proposed assay should be useful for on-site analysis of paraquat.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Forensic Toxicology, 31(2), pp.301-306; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本法中毒学会"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s11419-012-0175-0","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 Japanese Association of Forensic Toxicology and Springer Japan."},{"subitem_rights":"The final publication is available at link.springer.com."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18608965","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18608973","subitem_source_identifier_type":"ISSN"}]},"item_2_text_62":{"attribute_name":"出版者別言語","attribute_value_mlt":[{"subitem_text_value":"Japanese Association of Forensic Toxicology"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kishikawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Higuchi, Sho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohyama, Kaname"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakashima, Kenichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroda, Naotaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"ForTox31_301.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ForTox31_301.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/6478/files/ForTox31_301.pdf"},"version_id":"c63ca192-e708-4a94-8a64-f17db4cd0f10"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Beverage samples","subitem_subject_scheme":"Other"},{"subitem_subject":"Chemiluminescence","subitem_subject_scheme":"Other"},{"subitem_subject":"On-site analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"Paraquat","subitem_subject_scheme":"Other"},{"subitem_subject":"Portable luminometer","subitem_subject_scheme":"Other"},{"subitem_subject":"Redox cycle","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A simple and rapid chemiluminescence assay for on-site analysis of paraquat using a portable luminometer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A simple and rapid chemiluminescence assay for on-site analysis of paraquat using a portable luminometer"}]},"item_type_id":"2","owner":"2","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-26"},"publish_date":"2013-08-26","publish_status":"0","recid":"6478","relation_version_is_last":true,"title":["A simple and rapid chemiluminescence assay for on-site analysis of paraquat using a portable luminometer"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:44:31.784455+00:00"}