@article{oai:nagasaki-u.repo.nii.ac.jp:00006631, author = {Fathy, Bakr Ali Marwa and Kishikawa, Naoya and Ohyama, Kaname and Abdel-Mageed, Mohamed Horria and Mohamed, Abdel-Wadood Hanaa and Mohamed, Mohamed Ashraf and Kuroda, Naotaka}, journal = {Journal of Chromatography A}, month = {Jul}, note = {A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2'-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20. min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The detection limits were from 0.19 to 0.50. nM (1-10. fmol/injection) at a signal-to-noise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents., Journal of Chromatography A, 1300, pp.199-203; 2013}, pages = {199--203}, title = {Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2′-furil, a novel fluorogenic reagent}, volume = {1300}, year = {2013} }