{"created":"2023-05-15T16:34:09.435252+00:00","id":6659,"links":{},"metadata":{"_buckets":{"deposit":"773ff738-5fbb-4eb9-b11b-3471fb773f09"},"_deposit":{"created_by":2,"id":"6659","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"6659"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00006659","sets":["27:28"]},"author_link":["28053","28055","28049","28052","28054","28050","28051"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-07-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"14332","bibliographicPageStart":"14321","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"International Journal of Molecular Sciences"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Thrombospondin-1 (TSP-1), a matricellular protein widely acclaimed to be involved in the inhibition of angiogenesis and tumorigenesis, is synthesized and secreted by many cell types, including osteoblast and cancer cells. TSP-1 is highly upregulated during early stage of osteogenesis, whereas it inhibits terminal osteoblast differentiation. Expression of TSP-1 is downregulated in cancer cells, and its ectopic expression has been shown to restrain tumor growth. Transcriptional regulation of TSP-1 in osteogenesis and cancer is poorly understood; this prompted us to study its regulation by the two key regulators of the aforementioned processes: Runx2 and Runx3. Through a PCR-based cDNA subtraction technique, we identified and cloned a cDNA fragment for mouse TSP-1, whose expression was dramatically upregulated in response to Runx2 expression in mesenchymal stem cells. Moreover, TSP-1 expression was considerably reduced in the lung of Runx2 knockout mouse. On the other hand, TSP-1 gene expression drastically increased at both the transcriptional and translational levels in response to Runx3 expression in B16-F10 melanoma cells. In line with this, Runx2 and Runx3 bound to the TSP-1 promoter and stimulated its activity. Hence, these results provide first line of evidence that TSP-1 is a transcriptional target gene of Runx2 and Runx3.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"International Journal of Molecular Sciences, 14(7), pp.14321-14332; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Multidisciplinary Digital Publishing Institute (MDPI)"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/ijms140714321","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"14220067","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shi, Xiuming"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Deepak, Vishwa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Linghui"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ba, Xueqing"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Komori, Toshihisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zeng, Xianlu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Liu, Wenguang"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"IJMS14_14321.pdf","filesize":[{"value":"879.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"IJMS14_14321.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/6659/files/IJMS14_14321.pdf"},"version_id":"c128c122-6fb1-4d76-aabe-70e58ec6dcf9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Angiogenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"B16-F10","subitem_subject_scheme":"Other"},{"subitem_subject":"C3H10T1/2 stem cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Cancer","subitem_subject_scheme":"Other"},{"subitem_subject":"Melanoma","subitem_subject_scheme":"Other"},{"subitem_subject":"Metastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblast","subitem_subject_scheme":"Other"},{"subitem_subject":"Runx2","subitem_subject_scheme":"Other"},{"subitem_subject":"Runx3","subitem_subject_scheme":"Other"},{"subitem_subject":"TSP-1","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Thrombospondin-1 Is a Putative Target Gene of Runx2 and Runx3","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Thrombospondin-1 Is a Putative Target Gene of Runx2 and Runx3"}]},"item_type_id":"2","owner":"2","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-09-12"},"publish_date":"2013-09-12","publish_status":"0","recid":"6659","relation_version_is_last":true,"title":["Thrombospondin-1 Is a Putative Target Gene of Runx2 and Runx3"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:40:26.635559+00:00"}