@article{oai:nagasaki-u.repo.nii.ac.jp:00006759,
 author = {Sakai, Yusuke and Koike, Makiko and Hasegawa, Hideko and Yamanouchi, Kosho and Soyama, Akihiko and Takatsuki, Mitsuhisa and Kuroki, Tamotsu and Ohashi, Kazuo and Okano, Teruo and Eguchi, Susumu},
 issue = {7},
 journal = {PLoS ONE},
 month = {Jul},
 note = {Cell sheet engineering is attracting attention from investigators in various fields, from basic research scientists to clinicians focused on regenerative medicine. However, hepatocytes have a limited proliferation potential in vitro, and it generally takes a several days to form a sheet morphology and multi-layered sheets. We herein report our rapid and efficient technique for generating multi-layered human hepatic cell (HepaRG® cell) sheets using pre-cultured fibroblast monolayers derived from human skin (TIG-118 cells) as a feeder layer on a temperature-responsive culture dish. Multi-layered TIG-118/HepaRG cell sheets with a thick morphology were harvested on day 4 of culturing HepaRG cells by forceful contraction of the TIG-118 cells, and the resulting sheet could be easily handled. In addition, the human albumin and alpha 1-antitrypsin synthesis activities of TIG-118/HepaRG cells were approximately 1.2 and 1.3 times higher than those of HepaRG cells, respectively. Therefore, this technique is considered to be a promising modality for rapidly fabricating multi-layered human hepatocyte sheets from cells with limited proliferation potential, and the engineered cell sheet could be used for cell transplantation with highly specific functions., PLoS ONE, 8(7), e70970; 2013},
 title = {Rapid Fabricating Technique for Multi-Layered Human Hepatic Cell Sheets by Forceful Contraction of the Fibroblast Monolayer},
 volume = {8},
 year = {2013}
}