{"created":"2023-05-15T16:34:13.756564+00:00","id":6759,"links":{},"metadata":{"_buckets":{"deposit":"fd8d3bed-dc9c-4c30-bb4e-285d24595b95"},"_deposit":{"created_by":2,"id":"6759","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"6759"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00006759","sets":["29:30"]},"author_link":["28381","28376","28374","28375","28378","28377","28372","28379","28373","28380"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-07-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageStart":"e70970","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cell sheet engineering is attracting attention from investigators in various fields, from basic research scientists to clinicians focused on regenerative medicine. However, hepatocytes have a limited proliferation potential in vitro, and it generally takes a several days to form a sheet morphology and multi-layered sheets. We herein report our rapid and efficient technique for generating multi-layered human hepatic cell (HepaRG® cell) sheets using pre-cultured fibroblast monolayers derived from human skin (TIG-118 cells) as a feeder layer on a temperature-responsive culture dish. Multi-layered TIG-118/HepaRG cell sheets with a thick morphology were harvested on day 4 of culturing HepaRG cells by forceful contraction of the TIG-118 cells, and the resulting sheet could be easily handled. In addition, the human albumin and alpha 1-antitrypsin synthesis activities of TIG-118/HepaRG cells were approximately 1.2 and 1.3 times higher than those of HepaRG cells, respectively. Therefore, this technique is considered to be a promising modality for rapidly fabricating multi-layered human hepatocyte sheets from cells with limited proliferation potential, and the engineered cell sheet could be used for cell transplantation with highly specific functions.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"PLoS ONE, 8(7), e70970; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0070970","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 Sakai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakai, Yusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koike, Makiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasegawa, Hideko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamanouchi, Kosho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Soyama, Akihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takatsuki, Mitsuhisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuroki, Tamotsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohashi, Kazuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okano, Teruo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Eguchi, Susumu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"PLoS8_70970.pdf","filesize":[{"value":"9.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PLoS8_70970.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/6759/files/PLoS8_70970.pdf"},"version_id":"f6f2473e-c8be-4bbb-bba9-fc8d837f9b9b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Rapid Fabricating Technique for Multi-Layered Human Hepatic Cell Sheets by Forceful Contraction of the Fibroblast Monolayer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rapid Fabricating Technique for Multi-Layered Human Hepatic Cell Sheets by Forceful Contraction of the Fibroblast Monolayer"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-09-20"},"publish_date":"2013-09-20","publish_status":"0","recid":"6759","relation_version_is_last":true,"title":["Rapid Fabricating Technique for Multi-Layered Human Hepatic Cell Sheets by Forceful Contraction of the Fibroblast Monolayer"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:38:30.428801+00:00"}