{"created":"2023-05-15T16:34:19.432970+00:00","id":6889,"links":{},"metadata":{"_buckets":{"deposit":"e75466fa-a5b6-43e0-996a-56622832c58b"},"_deposit":{"created_by":2,"id":"6889","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"6889"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00006889","sets":["10:11"]},"author_link":["28880","28882","28879","28881"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-08-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"996","bibliographicPageStart":"989","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"Thyroid"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Epithelial-to-mesenchymal transition (EMT) is thought to play a critical role in the invasion and metastasis of cancer and to be associated with cancer stem cell (CSC) properties. It is not clear if there is a link between EMT and CSCs in thyroid cancers. We therefore investigated the CSC properties of thyroid cancers that underwent EMT. Method: To induce EMT (spindle-like cell morphology, loss and acquisition of expression of an epithelial marker E-cadherin and a mesenchymal marker vimentin respectively) in an epithelial-type thyroid cancer cell line ACT-1, we used transforming growth factor-β (TGF-β), BRAFV600E, and/or Snail homolog 1 (SNAI1, also known as SNAIL). CSC properties were analyzed with assays for cell proliferation, chemosensitivity, in vitro and in vivo tumor formation ability, cell surface antigens, and intracellular aldehyde dehydrogenase (ALDH; a known CSC marker) activities. Results: EMT was induced most efficiently by SNAIL (ACT-SNAIL cells), whereas TGF-β and BRAFV600E were less efficient. ACT-SNAIL cells showed slightly but significantly enhanced tumor formation ability in an in vitro sphere assay (approximately 3-fold) but not an in vivo subcutaneous tumor growth assay, and showed comparable chemosensitivity compared with the parental ACT-1 cells. However, of interest, although the in vitro sphere-formation ability of ALDH+ cells was almost unchanged after SNAIL induction, SNAIL overexpression induced much higher (approximately 14-fold) spheres in ALDH- cells. Thus, ALDH was no longer a CSC marker in ACT-SNAIL cells. Conclusions: All these data indicate that EMT confers CSC properties in ALDH- cells and appears to influence the ability of ALDH to enrich CSCs.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Thyroid, 23(8), pp.989-996; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Mary Ann Liebert Inc."}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1089/thy.2012.0319","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is a copy of an article published in the Thyroid © 2013 copyright Mary Ann Liebert, Inc.; Thyroid is available online at: http://online.liebertpub.com."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10507256","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"15579077","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yasui, Kazuaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimamura, Mika"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitsutake, Norisato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagayama, Yuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"Thy23_989.pdf","filesize":[{"value":"635.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Thy23_989.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/6889/files/Thy23_989.pdf"},"version_id":"3de6c9c1-6659-49ff-90c4-e142b60303d0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"SNAIL Induces Epithelial-to-Mesenchymal Transition and Cancer Stem Cell–Like Properties in Aldehyde Dehydroghenase–Negative Thyroid Cancer Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"SNAIL Induces Epithelial-to-Mesenchymal Transition and Cancer Stem Cell–Like Properties in Aldehyde Dehydroghenase–Negative Thyroid Cancer Cells"}]},"item_type_id":"2","owner":"2","path":["11"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-10-24"},"publish_date":"2013-10-24","publish_status":"0","recid":"6889","relation_version_is_last":true,"title":["SNAIL Induces Epithelial-to-Mesenchymal Transition and Cancer Stem Cell–Like Properties in Aldehyde Dehydroghenase–Negative Thyroid Cancer Cells"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:36:26.977764+00:00"}