{"created":"2023-05-15T16:34:20.509267+00:00","id":6909,"links":{},"metadata":{"_buckets":{"deposit":"eac860a6-2eba-4657-9c68-958c668e8b7f"},"_deposit":{"created_by":2,"id":"6909","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"6909"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00006909","sets":["29:30"]},"author_link":["28985","28994","28989","28992","28991","28987","28990","28986","28988","28993"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"501","bibliographicPageStart":"489","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"Cellular and Molecular Neurobiology"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Glioblastoma multiforme (GBM) cells invade along the existing normal capillaries in brain. Normal capillary endothelial cells function as the blood-brain barrier (BBB) that limits permeability of chemicals into the brain. To investigate whether GBM cells modulate the BBB function of normal endothelial cells, we developed a new in vitro BBB model with primary cultures of rat brain endothelial cells (RBECs), pericytes, and astrocytes. Cells were plated on a membrane with 8 μm pores, either as a monolayer or as a BBB model with triple layer culture. The BBB model consisted of RBEC on the luminal side as a bottom, and pericytes and astrocytes on the abluminal side as a top of the chamber. Human GBM cell line, LN-18 cells, or lung cancer cell line, NCI-H1299 cells, placed on either the RBEC monolayer or the BBB model increased the transendothelial electrical resistance (TEER) values against the model, which peaked within 72 h after the tumor cell application. The TEER value gradually returned to baseline with LN-18 cells, whereas the value quickly dropped to the baseline in 24 h with NCI-H1299 cells. NCI-H1299 cells invaded into the RBEC layer through the membrane, but LN-18 cells did not. Fibroblast growth factor 2 (FGF-2) strengthens the endothelial cell BBB function by increased occludin and ZO-1 expression. In our model, LN-18 and NCI-H1299 cells secreted FGF-2, and a neutralization antibody to FGF-2 inhibited LN-18 cells enhanced BBB function. These results suggest that FGF-2 would be a novel therapeutic target for GBM in the perivascular invasive front.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Cellular and Molecular Neurobiology, 33(4), pp.489-501; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer New York"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s10571-013-9913-z","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2013 Springer Science+Business Media New York."},{"subitem_rights":"The final publication is available at link.springer.com"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02724340","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_8":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"15736830","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Toyoda, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Kunihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Shinsuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Thuy, Dinh Ha Duy"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ujifuku, Kenta"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamada, Kensaku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Kentaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuo, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagata, Izumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niwa, Masami"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"CMN33_489.pdf","filesize":[{"value":"588.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CMN33_489.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/6909/files/CMN33_489.pdf"},"version_id":"76377b39-5eed-4b49-8b91-92b39970788e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Blood-brain barrier","subitem_subject_scheme":"Other"},{"subitem_subject":"Fibroblast growth factor 2","subitem_subject_scheme":"Other"},{"subitem_subject":"Glioblastoma","subitem_subject_scheme":"Other"},{"subitem_subject":"Rat brain endothelial cells","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Initial Contact of Glioblastoma Cells with Existing Normal Brain Endothelial Cells Strengthen the Barrier Function via Fibroblast Growth Factor 2 Secretion: A New In Vitro Blood–Brain Barrier Model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Initial Contact of Glioblastoma Cells with Existing Normal Brain Endothelial Cells Strengthen the Barrier Function via Fibroblast Growth Factor 2 Secretion: A New In Vitro Blood–Brain Barrier Model"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-06-01"},"publish_date":"2014-06-01","publish_status":"0","recid":"6909","relation_version_is_last":true,"title":["Initial Contact of Glioblastoma Cells with Existing Normal Brain Endothelial Cells Strengthen the Barrier Function via Fibroblast Growth Factor 2 Secretion: A New In Vitro Blood–Brain Barrier Model"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:36:13.365796+00:00"}