{"created":"2023-05-15T16:34:20.636577+00:00","id":6911,"links":{},"metadata":{"_buckets":{"deposit":"a8bda71b-440e-41b6-9a64-1f71aef5292d"},"_deposit":{"created_by":2,"id":"6911","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"6911"},"status":"published"},"_oai":{"id":"oai:nagasaki-u.repo.nii.ac.jp:00006911","sets":["29:30"]},"author_link":["29007","29009","29010","29005","29004","29008","29006"],"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"3834","bibliographicPageStart":"3825","bibliographicVolumeNumber":"81","bibliographic_titles":[{"bibliographic_title":"Infection and Immunity"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Following Anopheles mosquito-mediated introduction into a human host, Plasmodium parasites infect hepatocytes and undergo intensive replication. Accumulating evidence indicates that CD8+ T cells induced by immunization with attenuated Plasmodium sporozoites can confer sterile immunity at the liver stage of infection; however, the mechanisms underlying this protection are not clearly understood. To address this, we generated recombinant Plasmodium berghei ANKA expressing a fusion protein of an ovalbumin epitope and green fluorescent protein in the cytoplasm of the parasite. We have shown that the ovalbumin epitope is presented by infected liver cells in a manner dependent on a transporter associated with antigen processing and becomes a target of specific CD8+ T cells from the T cell receptor transgenic mouse line OT-I, leading to protection at the liver stage of Plasmodium infection. We visualized the interaction between OT-I cells and infected hepatocytes by intravital imaging using two-photon microscopy. OT-I cells formed clusters around infected hepatocytes, leading to the elimination of the intrahepatic parasites and subsequent formation of large clusters of OT-I cells in the liver. Gamma interferon expressed in CD8+ T cells was dispensable for this protective response. Additionally, we found that polyclonal ovalbumin-specific memory CD8+ T cells induced by de novo immunization were able to confer sterile protection, although the threshold frequency of the protection was relatively high. These studies revealed a novel mechanism of specific CD8+ T cell-mediated protective immunity and demonstrated that proteins expressed in the cytoplasm of Plasmodium parasites can become targets of specific CD8+ T cells during liver-stage infection.","subitem_description_type":"Abstract"}]},"item_2_description_63":{"attribute_name":"引用","attribute_value_mlt":[{"subitem_description":"Infection and Immunity, 81(10), pp.3825-3834; 2013","subitem_description_type":"Other"}]},"item_2_publisher_33":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_2_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1128/IAI.00570-13","subitem_relation_type_select":"DOI"}}]},"item_2_rights_13":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2013, American Society for Microbiology."}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00199567","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kimura, Kazumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsushima, Yoshifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyakoda, Mana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honma, Kiri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yuda, Masao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yui, Katsuyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-21"}],"displaytype":"detail","filename":"InfImm81_3825.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"InfImm81_3825.pdf","url":"https://nagasaki-u.repo.nii.ac.jp/record/6911/files/InfImm81_3825.pdf"},"version_id":"424b8fe8-d3b9-459b-ad5d-2645257b117c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"CD8+ T Cells Specific for a Malaria Cytoplasmic Antigen Form Clusters around Infected Hepatocytes and Are Protective at the Liver Stage of Infection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"CD8+ T Cells Specific for a Malaria Cytoplasmic Antigen Form Clusters around Infected Hepatocytes and Are Protective at the Liver Stage of Infection"}]},"item_type_id":"2","owner":"2","path":["30"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-05-01"},"publish_date":"2014-05-01","publish_status":"0","recid":"6911","relation_version_is_last":true,"title":["CD8+ T Cells Specific for a Malaria Cytoplasmic Antigen Form Clusters around Infected Hepatocytes and Are Protective at the Liver Stage of Infection"],"weko_creator_id":"2","weko_shared_id":-1},"updated":"2023-05-16T02:36:11.022495+00:00"}